Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity

The New World arenavirus Junin (JUNV) is the etiological agent of Argentine hemorrhagic fever (AHF). Previous studies of human macrophage infection by the Old-World arenaviruses Mopeia and Lassa showed that while the non-pathogenic Mopeia virus replicates and activates human macrophages, the pathoge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ferrer, María Florencia, Thomas, Pablo, López Ortiz, Aída Oryza, Errasti, Andrea E., Charo, Nancy, Romanowski, Víctor, Gorgojo, Juan Pablo, Rodríguez, María Eugenia, Carrera Silva, Eugenio A., Gómez, Ricardo Martín
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Ciencias Exactas Biología Ciencias Médicas junin virus human macrophages TAM receptors macrophage activation macrophage polarization IFN-I
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107682 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6817498&blobtype=pdf https://www.frontiersin.org/articles/10.3389/fimmu.2019.02499/full
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107682
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Biología Ciencias Médicas junin virus human macrophages TAM receptors macrophage activation macrophage polarization IFN-I
spellingShingle	Ciencias Exactas Biología Ciencias Médicas junin virus human macrophages TAM receptors macrophage activation macrophage polarization IFN-I Ferrer, María Florencia Thomas, Pablo López Ortiz, Aída Oryza Errasti, Andrea E. Charo, Nancy Romanowski, Víctor Gorgojo, Juan Pablo Rodríguez, María Eugenia Carrera Silva, Eugenio A. Gómez, Ricardo Martín Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity
topic_facet	Ciencias Exactas Biología Ciencias Médicas junin virus human macrophages TAM receptors macrophage activation macrophage polarization IFN-I
description	The New World arenavirus Junin (JUNV) is the etiological agent of Argentine hemorrhagic fever (AHF). Previous studies of human macrophage infection by the Old-World arenaviruses Mopeia and Lassa showed that while the non-pathogenic Mopeia virus replicates and activates human macrophages, the pathogenic Lassa virus replicates but fails to activate human macrophages. Less is known in regard to the impact of New World arenavirus infection on the human macrophage immune response. Macrophage activation is critical for controlling infections but could also be usurped favoring immune evasion. Therefore, it is crucial to understand how the JUNV infection modulates macrophage plasticity to clarify its role in AHF pathogenesis. With this aim in mind, we compared infection with the attenuated Candid 1 (C#1) or the pathogenic P strains of the JUNV virus in human macrophage cultures. The results showed that both JUNV strains similarly replicated and induced morphological changes as early as 1 day post-infection. However, both strains differentially induced the expression of CD71, the receptor for cell entry, the activation and maturation molecules CD80, CD86, and HLA-DR and selectively modulated cytokine production. Higher levels of TNF-α, IL-10, and IL-12 were detected with C#1 strain, while the P strain induced only higher levels of IL-6. We also found that C#1 strain infection skewed macrophage polarization to M1, whereas the P strain shifted the response to an M2 phenotype. Interestingly, the MERTK receptor, that negatively regulates the immune response, was down-regulated by C#1 strain and up-regulated by P strain infection. Similarly, the target genes of MERTK activation, the cytokine suppressors SOCS1 and SOCS3, were also increased after P strain infection, in addition to IRF-1, that regulates type I IFN levels, which were higher with C#1 compared with P strain infection. Together, this differential activation/polarization pattern of macrophages elicited by P strain suggests a more evasive immune response and may have important implications in the pathogenesis of AHF and underpinning the development of new potential therapeutic strategies
format	Articulo Articulo
author	Ferrer, María Florencia Thomas, Pablo López Ortiz, Aída Oryza Errasti, Andrea E. Charo, Nancy Romanowski, Víctor Gorgojo, Juan Pablo Rodríguez, María Eugenia Carrera Silva, Eugenio A. Gómez, Ricardo Martín
author_facet	Ferrer, María Florencia Thomas, Pablo López Ortiz, Aída Oryza Errasti, Andrea E. Charo, Nancy Romanowski, Víctor Gorgojo, Juan Pablo Rodríguez, María Eugenia Carrera Silva, Eugenio A. Gómez, Ricardo Martín
author_sort	Ferrer, María Florencia
title	Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity
title_short	Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity
title_full	Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity
title_fullStr	Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity
title_full_unstemmed	Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity
title_sort	junin virus triggers macrophage activation and modulates polarization according to viral strain pathogenicity
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/107682 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6817498&blobtype=pdf https://www.frontiersin.org/articles/10.3389/fimmu.2019.02499/full
work_keys_str_mv	AT ferrermariaflorencia juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT thomaspablo juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT lopezortizaidaoryza juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT errastiandreae juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT charonancy juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT romanowskivictor juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT gorgojojuanpablo juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT rodriguezmariaeugenia juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT carrerasilvaeugenioa juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity AT gomezricardomartin juninvirustriggersmacrophageactivationandmodulatespolarizationaccordingtoviralstrainpathogenicity
bdutipo_str	Repositorios
_version_	1764820443495137282

Junin Virus Triggers Macrophage Activation and Modulates Polarization According to Viral Strain Pathogenicity

Ejemplares similares