Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice

Integrity of the thymus during perinatal life is necessary for a proper maturation of the pituitarygonadal axis in mice and other mammalian species. Thus congenitally athymic (nude) female mice show significantly reduced levels of circulating gonadotropins, a fact that seems to be causally related t...

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Autores principales: Goya, Rodolfo Gustavo, Reggiani, Paula Cecilia, Vesenbeckh, Silvan M, Pléau, Jean M, Sosa, Yolanda Elena, Cónsole-Avegliano, Gloria Miriam, Schade, Rüdiger, Henklein, Peter, Dardenne, Mireille
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2007
Materias:
Bioquímica
Reproductive derangements
Thymulin immunoneutralization
Synthetic gene
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107681
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2656608&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107681
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Reproductive derangements
Thymulin immunoneutralization
Synthetic gene
spellingShingle Bioquímica
Reproductive derangements
Thymulin immunoneutralization
Synthetic gene
Goya, Rodolfo Gustavo
Reggiani, Paula Cecilia
Vesenbeckh, Silvan M
Pléau, Jean M
Sosa, Yolanda Elena
Cónsole-Avegliano, Gloria Miriam
Schade, Rüdiger
Henklein, Peter
Dardenne, Mireille
Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
topic_facet Bioquímica
Reproductive derangements
Thymulin immunoneutralization
Synthetic gene
description Integrity of the thymus during perinatal life is necessary for a proper maturation of the pituitarygonadal axis in mice and other mammalian species. Thus congenitally athymic (nude) female mice show significantly reduced levels of circulating gonadotropins, a fact that seems to be causally related to a number of reproductive derangements described in these mutants. Interestingly, a number of in vitro studies suggest that the thymic peptide thymulin may be involved in thymus-pituitary communication. To determine the consequences of low serum thymulin in otherwise normal animals, we induced short (8 days)- and long (33 days)-term thymulin deficiency in C57BL/6 mice by neonatally injecting (intraperitoneally) an anti-thymulin serum and assessed their circulating gonadotropin levels at puberty and thereafter. Control mice received an irrelevant antiserum. Gonadotropins were measured by radioimmunoassay and thymulin by bioassay. Both long- and short-term serum thymulin immunoneutralization resulted in a significant reduction in the serum levels of gonadotropins at 33 and 45 days of age. Subsequently, we injected (intramuscularly) an adenoviral vector harboring a synthetic DNA sequence (5′- ATGCAAGCCAAATCTCAAGGTGGATCCAACTAGTAG-3′) encoding a biologically active analog of thymulin, methionine-FTS, in newborn nude mice (which are thymulin deficient) and measured circulating gonadotropin levels when the animals reached 52 days of age. It was observed that neonatal thymulin gene therapy in the athymic mice restored their serum thymulin levels and prevented the reduction in circulating gonadotropin levels that typically emerges in these mutants after puberty. Our results indicate that thymulin plays a relevant physiological role in the thymuspituitary-gonadal axis.
format Articulo
Preprint
author Goya, Rodolfo Gustavo
Reggiani, Paula Cecilia
Vesenbeckh, Silvan M
Pléau, Jean M
Sosa, Yolanda Elena
Cónsole-Avegliano, Gloria Miriam
Schade, Rüdiger
Henklein, Peter
Dardenne, Mireille
author_facet Goya, Rodolfo Gustavo
Reggiani, Paula Cecilia
Vesenbeckh, Silvan M
Pléau, Jean M
Sosa, Yolanda Elena
Cónsole-Avegliano, Gloria Miriam
Schade, Rüdiger
Henklein, Peter
Dardenne, Mireille
author_sort Goya, Rodolfo Gustavo
title Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
title_short Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
title_full Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
title_fullStr Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
title_full_unstemmed Thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
title_sort thymulin gene therapy prevents the reduction in circulating gonadotropins induced by thymulin deficiency in mice
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/107681
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2656608&blobtype=pdf
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