Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study

The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of <i>Giardia intestinalis</i> strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evalu...

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Autores principales:	Pérez, Pablo Fernando, Minnaard, Jessica, Rouvet, Martine, Knabenhans, Christian, Brassart, Dominique, De Antoni, Graciela Liliana, Schiffrin, Eduardo J.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2001
Materias:	Ciencias Exactas Giardia intestinalis in vitro study
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/107655 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC93268&blobtype=pdf https://aem.asm.org/content/67/11/5037
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-107655
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Giardia intestinalis in vitro study
spellingShingle	Ciencias Exactas Giardia intestinalis in vitro study Pérez, Pablo Fernando Minnaard, Jessica Rouvet, Martine Knabenhans, Christian Brassart, Dominique De Antoni, Graciela Liliana Schiffrin, Eduardo J. Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
topic_facet	Ciencias Exactas Giardia intestinalis in vitro study
description	The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of <i>Giardia intestinalis</i> strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. <i>Lactobacillus johnsonii</i> La1 significantly inhibited the proliferation of <i>G. intestinalis</i> trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G<sub>1</sub> phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of <i>Giardia</i> trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis <i>in vivo</i>.
format	Articulo Articulo
author	Pérez, Pablo Fernando Minnaard, Jessica Rouvet, Martine Knabenhans, Christian Brassart, Dominique De Antoni, Graciela Liliana Schiffrin, Eduardo J.
author_facet	Pérez, Pablo Fernando Minnaard, Jessica Rouvet, Martine Knabenhans, Christian Brassart, Dominique De Antoni, Graciela Liliana Schiffrin, Eduardo J.
author_sort	Pérez, Pablo Fernando
title	Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_short	Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_full	Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_fullStr	Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_full_unstemmed	Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_sort	inhibition of <i>giardia intestinalis</i> by extracellular factors from lactobacilli: an in vitro study
publishDate	2001
url	http://sedici.unlp.edu.ar/handle/10915/107655 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC93268&blobtype=pdf https://aem.asm.org/content/67/11/5037
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bdutipo_str	Repositorios
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Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study

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