Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters

In the present study, the toxicity of receiving waters from a highly polluted urban watercourse, the Reconquista River, Argentina, collected at a dam in the upstream part of the river was evaluated. <i>Cnesterodon decemmaculatus</i>, a widely distributed fsh species in Pampasic rivers pr...

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Autores principales: Ossana, Natalia Alejandra, Baudou, Federico Gastón, Castañé, Patricia Mónica, Tripoli, Luis, Soloneski, Sonia María Elsa, Ferrari, Lucrecia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Naturales
Cnesterodon decemmaculatus
toxicity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107649
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6332968&blobtype=pdf
https://www.hindawi.com/journals/jt/2019/4687685/
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107649
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Cnesterodon decemmaculatus
toxicity
spellingShingle Ciencias Naturales
Cnesterodon decemmaculatus
toxicity
Ossana, Natalia Alejandra
Baudou, Federico Gastón
Castañé, Patricia Mónica
Tripoli, Luis
Soloneski, Sonia María Elsa
Ferrari, Lucrecia
Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters
topic_facet Ciencias Naturales
Cnesterodon decemmaculatus
toxicity
description In the present study, the toxicity of receiving waters from a highly polluted urban watercourse, the Reconquista River, Argentina, collected at a dam in the upstream part of the river was evaluated. <i>Cnesterodon decemmaculatus</i>, a widely distributed fsh species in Pampasic rivers proposed for use in ecotoxicological evaluations, was used as a test organism. A 96-h acute toxicity bioassay with river water quality which has been characterized as moderately contaminated was performed. Te treatment groups were (1) whole surface river water; (2) whole surface river water with 2 mg Cd/L added as a simulated metal contaminant pulse; (3) a negative control using reconstituted moderately hard water (MHW); (4) a metal positive control, MHW + 2 mg Cd/L; and (5) a positive genotoxicity control, MHW + 5 mg Cyclophosphamide/L (CP). Te condition factor rate, micronuclei frequency, and comet assay from peripherical blood, structural changes of the gill arrangement by scanning electron microscope (SEM) analysis, histopathological changes in the liver and the glutathione-S-transferase, catalase, superoxide dismutase, glutathione, and protein content from the body midsection (viscera) were evaluated. According to our results, for short term exposure, SEM analyses of gills and liver histopathological analyses could be useful tools for the evaluation of target organ damage as well as comet assays for DNA damage. We propose that the 96-h laboratory bioassay protocol described is useful for monitoring the deterioration of water quality employing the teleost <i>C. decemmaculatus</i> and that the microscope analysis of gills and liver as well as the comet assay methodology could be sensitive endpoint indicators.
format Articulo
Articulo
author Ossana, Natalia Alejandra
Baudou, Federico Gastón
Castañé, Patricia Mónica
Tripoli, Luis
Soloneski, Sonia María Elsa
Ferrari, Lucrecia
author_facet Ossana, Natalia Alejandra
Baudou, Federico Gastón
Castañé, Patricia Mónica
Tripoli, Luis
Soloneski, Sonia María Elsa
Ferrari, Lucrecia
author_sort Ossana, Natalia Alejandra
title Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters
title_short Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters
title_full Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters
title_fullStr Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters
title_full_unstemmed Histological, Genotoxic, and Biochemical Effects on <i>Cnesterodon decemmaculatus</i> (Jenyns 1842) (Cyprinodontiformes, Poeciliidae): Early Response Bioassays to Assess the Impact of Receiving Waters
title_sort histological, genotoxic, and biochemical effects on <i>cnesterodon decemmaculatus</i> (jenyns 1842) (cyprinodontiformes, poeciliidae): early response bioassays to assess the impact of receiving waters
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/107649
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6332968&blobtype=pdf
https://www.hindawi.com/journals/jt/2019/4687685/
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