Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses

Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular tran...

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Detalles Bibliográficos
Autores principales: Fiorentino, María Andrea, Paolicchi, Fernando Alberto, Campero, Carlos Manuel, Barbeito, Claudio Gustavo
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Bovine fetuses
Brucella abortus
Immunohistochemical
Lectin
Placenta
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107594
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5918125&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107594
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Bovine fetuses
Brucella abortus
Immunohistochemical
Lectin
Placenta
spellingShingle Ciencias Veterinarias
Bovine fetuses
Brucella abortus
Immunohistochemical
Lectin
Placenta
Fiorentino, María Andrea
Paolicchi, Fernando Alberto
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses
topic_facet Ciencias Veterinarias
Bovine fetuses
Brucella abortus
Immunohistochemical
Lectin
Placenta
description Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses.
format Articulo
Comunicacion
author Fiorentino, María Andrea
Paolicchi, Fernando Alberto
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author_facet Fiorentino, María Andrea
Paolicchi, Fernando Alberto
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author_sort Fiorentino, María Andrea
title Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses
title_short Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses
title_full Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses
title_fullStr Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses
title_full_unstemmed Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>Brucella abortus</i>-bovine infected fetuses
title_sort lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of <i>brucella abortus</i>-bovine infected fetuses
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/107594
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5918125&blobtype=pdf
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