Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study
Background: The sweetener and hypoglycemic properties of stevioside (STV) are well known, as the main component of the plant <i>Stevia rebaudiana</i>. Given its extensive use in diabetic patients, it was of interest to evaluate its effects on the most frequent cardiovascular disease, the...
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| Autores principales: | , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/107560 https://www.sciencedirect.com/science/article/abs/pii/S0944711317301125 |
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I19-R120-10915-107560 |
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dspace |
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Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología calorimetry ischemia/reperfusion heart stevioside cellular Ca2+ |
| spellingShingle |
Ciencias Exactas Biología calorimetry ischemia/reperfusion heart stevioside cellular Ca2+ Ragone, María Inés Bonazzola, Patricia Colareda, Germán Andrés Lazarte, María Lara Bruno, Fiorella Gianina Consolini, Alicia Elvira Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study |
| topic_facet |
Ciencias Exactas Biología calorimetry ischemia/reperfusion heart stevioside cellular Ca2+ |
| description |
Background: The sweetener and hypoglycemic properties of stevioside (STV) are well known, as the main component of the plant <i>Stevia rebaudiana</i>. Given its extensive use in diabetic patients, it was of interest to evaluate its effects on the most frequent cardiovascular disease, the coronary insufficiency.
Purpose: To study whether STV could be cardioprotective against ischemia-reperfusion (I/R) in a model of “stunning” in rat hearts.
Study design: A preclinical study was performed in isolated hearts from rats in the following groups: non-treated rats whose hearts were perfused with STV 0.3 mg/ml and their controls (C) exposed to either moderate stunning (20 min I/45 min R) or severe stunning (30 min I/45 min R), and a group of rats orally treated with STV 25 mg/kg/day in the drink water during 1 week before the experiment of severe stunning in the isolated hearts were done.
Methods: The mechano-calorimetrical performance of isolated beating hearts was recorded during stabilization period with control Krebs perfusion inside a calorimeter, with or without 0.3 mg/ml STV before the respective period of I/R. The left ventricular maximal developed pressure (P) and total heat rate (Ht) were continuously measured.
Results: Both, orally administered and perfused STV improved the post-ischemic contractile recovery (PICR, as % of initial control P) and the total muscle economy (P/Ht) after the severe stunning, but only improved P/Ht in moderate stunning. However, STV increased the diastolic pressure (LVEDP) during I/R in both stunning models. For studying the mechanism of action, ischemic hearts were reperfused with 10 mM caffeine-36 mM Na+-Krebs to induce a contracture dependent on sarcorreticular Ca<sup>2+</sup> content, whose relaxation mainly depends on mitochondrial Ca<sup>2+</sup> uptake. STV at 0.3 mg/ml increased the area-under-curve of the caffeine-dependent contracture (AUC-LVP). Moreover, at room temperature STV increased the mitochondrial Ca<sup>2+</sup> uptake measured by Rhod-2 fluorescence in rat cardiomyocytes, but prevented the [Ca<sup>2+</sup>]m overload assessed by caffeine-dependent SR release.
Conclusions: Results suggest that STV is cardioprotective against I/R under oral administration or direct perfusion in hearts. The mechanism includes the regulation of the myocardial calcium homeostasis and the energetic during I/R in several sites, mainly reducing mitochondrial Ca<sup>2+</sup> overload and increasing the sarcorreticular Ca<sup>2+</sup> store. |
| format |
Articulo Articulo |
| author |
Ragone, María Inés Bonazzola, Patricia Colareda, Germán Andrés Lazarte, María Lara Bruno, Fiorella Gianina Consolini, Alicia Elvira |
| author_facet |
Ragone, María Inés Bonazzola, Patricia Colareda, Germán Andrés Lazarte, María Lara Bruno, Fiorella Gianina Consolini, Alicia Elvira |
| author_sort |
Ragone, María Inés |
| title |
Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study |
| title_short |
Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study |
| title_full |
Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study |
| title_fullStr |
Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study |
| title_full_unstemmed |
Cardioprotection of stevioside on stunned rat hearts: A mechano-energetical study |
| title_sort |
cardioprotection of stevioside on stunned rat hearts: a mechano-energetical study |
| publishDate |
2017 |
| url |
http://sedici.unlp.edu.ar/handle/10915/107560 https://www.sciencedirect.com/science/article/abs/pii/S0944711317301125 |
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