Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy

In order to better understand the role of β-carotene and imidazole on the Photodynamic Therapy (PDT) mechanism, synchrotron infrared microscopywas used to detect the associated intracellular biochemical modifications following the visible light irradiation of HeLa cells incubated with these compound...

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Detalles Bibliográficos
Autores principales: Bosio, Gabriela Natalia, Parisi, Julieta Marcia, García Einschlag, Fernando Sebastián, Mártire, Daniel Osvaldo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Química
β-carotene
Imidazole
HeLa
HaCat
SKMEL-28 PDT
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107469
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107469
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
β-carotene
Imidazole
HeLa
HaCat
SKMEL-28 PDT
spellingShingle Química
β-carotene
Imidazole
HeLa
HaCat
SKMEL-28 PDT
Bosio, Gabriela Natalia
Parisi, Julieta Marcia
García Einschlag, Fernando Sebastián
Mártire, Daniel Osvaldo
Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
topic_facet Química
β-carotene
Imidazole
HeLa
HaCat
SKMEL-28 PDT
description In order to better understand the role of β-carotene and imidazole on the Photodynamic Therapy (PDT) mechanism, synchrotron infrared microscopywas used to detect the associated intracellular biochemical modifications following the visible light irradiation of HeLa cells incubated with these compounds as typical hydrophobic and hydrophilic singlet oxygen quenchers, respectively. For this purpose, PDT was performed employing the hydrophilic sensitizer 5,10,15,20-Tetrakis (1-methyl-4-pyridinio) porphyrin tetra (p-toluenesulfonate), TMPyP, and the hydrophobic sensitizer 5-(4-Methoxycarboxyphenyl)-10,15,20-triphenyl-21H,23H–porphyrin. The single cell IR spectra of PDT-treated, PDT plus quencher-treated and control HeLa cellswere recorded at the SOLEIL Synchrotron Infrared SMIS beamline targeting specifically the cell nucleus. Principal Component Analysis (PCA) was used to assess the IR spectral changes. PCA revealed that there is a frequency shift of the protein Amide I vibrational band for the assays with the TMPyP sensitizer, indicating changes in the protein secondary structures of the PDT-treated cancer cells compared to the controls. In addition, the scores in those cells treated with both quenchers appear to be similar to the controls indicating a photoprotective effect. Comparative experiments carried out with SKMEL-28 and HaCat cells showed non- significant photoprotective effects of β-carotene and imidazole.
format Articulo
Articulo
author Bosio, Gabriela Natalia
Parisi, Julieta Marcia
García Einschlag, Fernando Sebastián
Mártire, Daniel Osvaldo
author_facet Bosio, Gabriela Natalia
Parisi, Julieta Marcia
García Einschlag, Fernando Sebastián
Mártire, Daniel Osvaldo
author_sort Bosio, Gabriela Natalia
title Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
title_short Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
title_full Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
title_fullStr Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
title_full_unstemmed Imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
title_sort imidazole and beta-carotene photoprotection against photodynamic therapy evaluated by synchrotron infrared microscopy
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/107469
work_keys_str_mv AT bosiogabrielanatalia imidazoleandbetacarotenephotoprotectionagainstphotodynamictherapyevaluatedbysynchrotroninfraredmicroscopy
AT parisijulietamarcia imidazoleandbetacarotenephotoprotectionagainstphotodynamictherapyevaluatedbysynchrotroninfraredmicroscopy
AT garciaeinschlagfernandosebastian imidazoleandbetacarotenephotoprotectionagainstphotodynamictherapyevaluatedbysynchrotroninfraredmicroscopy
AT martiredanielosvaldo imidazoleandbetacarotenephotoprotectionagainstphotodynamictherapyevaluatedbysynchrotroninfraredmicroscopy
bdutipo_str Repositorios
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