Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms

The capacity of microorganisms from water kefir (WK) to control Aspergillus flavus growth during the aerobic phase of ensiled sorghum grains was determined. Sorghum inoculated with A. flavus was treated with filter-sterilized and non-sterilized water kefir, ensiled, and incubated 7 days at 25 ◦C. A....

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Autores principales: Gonda, Mariana, Garmendia, Gabriela, Rufo, Caterina, León Peláez, Ángela María, Wisniewski, Michael, Droby, Samir, Vero, Silvana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Biología
Water kefir
High moisture sorghum silage
Aspergillus flavus
High-throughput sequencing
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107429
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6722955&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107429
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Water kefir
High moisture sorghum silage
Aspergillus flavus
High-throughput sequencing
spellingShingle Biología
Water kefir
High moisture sorghum silage
Aspergillus flavus
High-throughput sequencing
Gonda, Mariana
Garmendia, Gabriela
Rufo, Caterina
León Peláez, Ángela María
Wisniewski, Michael
Droby, Samir
Vero, Silvana
Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms
topic_facet Biología
Water kefir
High moisture sorghum silage
Aspergillus flavus
High-throughput sequencing
description The capacity of microorganisms from water kefir (WK) to control Aspergillus flavus growth during the aerobic phase of ensiled sorghum grains was determined. Sorghum inoculated with A. flavus was treated with filter-sterilized and non-sterilized water kefir, ensiled, and incubated 7 days at 25 ◦C. A. flavus growth was quantified by qPCR after incubation. Mold growth was inhibited in the presence of water kefir while no inhibition was observed when filter-sterilized water kefir was applied, demonstrating the relevant role of the microorganisms in the kefir water in the biocontrol process. Fungal and bacterial diversity in treated sorghum mini-silos was analyzed by high-throughput sequencing. Firmicutes was the predominant bacterial phyla and Lactobacillus represented the most abundant genus, while Ascomycota was the predominant fungal phyla with Saccharomyces and Pichia as the major genera. Bacterial and yeast counts before and after incubation indicated that the microbial community obtained from WK was able to grow in the sorghum mini-silos in the presence of A. flavus. Results of the present work indicate that the use of a mixed inoculum of microorganisms present in WK may represent an alternative management practice to avoid the growth of A. flavus in ensiled sorghum grains and the concomitant contamination with aflatoxins.
format Articulo
Articulo
author Gonda, Mariana
Garmendia, Gabriela
Rufo, Caterina
León Peláez, Ángela María
Wisniewski, Michael
Droby, Samir
Vero, Silvana
author_facet Gonda, Mariana
Garmendia, Gabriela
Rufo, Caterina
León Peláez, Ángela María
Wisniewski, Michael
Droby, Samir
Vero, Silvana
author_sort Gonda, Mariana
title Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms
title_short Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms
title_full Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms
title_fullStr Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms
title_full_unstemmed Biocontrol of Aspergillus flavus in Ensiled Sorghum by Water Kefir Microorganisms
title_sort biocontrol of aspergillus flavus in ensiled sorghum by water kefir microorganisms
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/107429
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6722955&blobtype=pdf
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