Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation

Mesenchymal stem/stromal cells (MSCs) exhibit multidifferentiation potential, paralleled with immunomodulatory and trophic properties that make them viable alternative tools for the treatment of degenerative disorders, allograft rejection, autoimmune diseases, and tissue regeneration. MSC functional...

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Detalles Bibliográficos
Autores principales: Avila-Portillo, Luz M., Aristizabal, Fabio, Riveros, Angela, Abba, Martín Carlos, Correa, Diego
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
G-CSF
ADSC
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107402
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7044478&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107402
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
G-CSF
ADSC
spellingShingle Ciencias Médicas
G-CSF
ADSC
Avila-Portillo, Luz M.
Aristizabal, Fabio
Riveros, Angela
Abba, Martín Carlos
Correa, Diego
Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation
topic_facet Ciencias Médicas
G-CSF
ADSC
description Mesenchymal stem/stromal cells (MSCs) exhibit multidifferentiation potential, paralleled with immunomodulatory and trophic properties that make them viable alternative tools for the treatment of degenerative disorders, allograft rejection, autoimmune diseases, and tissue regeneration. MSC functional attributes can be modulated by exposing them to inflammatory-stimulating microenvironments (i.e., priming) before their therapeutic use. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that plays key roles in immune response and hematopoiesis modulation through direct effects on hematopoietic progenitors’ proliferation, survival, and mobilization. Despite the established roles of MSCs supporting hematopoiesis, the effects of G-CSF on MSCs biology have not been thoroughly explored. This study reveals that G-CSF has also direct effects on adipose-derived MSCs (ADSCs), modulating their functions. Herein, microarray-based transcriptomic analysis shows that G-CSF stimulation in vitro results in modulation of various signaling pathways including ones related with the metabolism of hyaluronan (HA), conferring a profile of cell mobilization to ADSCs, mediated in a cell-intrinsic fashion in part by reducing CD44 expression and HA synthesis-related genes. Collectively, these data suggest a direct modulatory effect of G-CSF on ADSC function, potentially altering their therapeutic capacity and thus the design of future clinical protocols.
format Articulo
Articulo
author Avila-Portillo, Luz M.
Aristizabal, Fabio
Riveros, Angela
Abba, Martín Carlos
Correa, Diego
author_facet Avila-Portillo, Luz M.
Aristizabal, Fabio
Riveros, Angela
Abba, Martín Carlos
Correa, Diego
author_sort Avila-Portillo, Luz M.
title Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation
title_short Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation
title_full Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation
title_fullStr Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation
title_full_unstemmed Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation
title_sort modulation of adipose-derived mesenchymal stem/stromal cell transcriptome by g-csf stimulation
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107402
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7044478&blobtype=pdf
work_keys_str_mv AT avilaportilloluzm modulationofadiposederivedmesenchymalstemstromalcelltranscriptomebygcsfstimulation
AT aristizabalfabio modulationofadiposederivedmesenchymalstemstromalcelltranscriptomebygcsfstimulation
AT riverosangela modulationofadiposederivedmesenchymalstemstromalcelltranscriptomebygcsfstimulation
AT abbamartincarlos modulationofadiposederivedmesenchymalstemstromalcelltranscriptomebygcsfstimulation
AT correadiego modulationofadiposederivedmesenchymalstemstromalcelltranscriptomebygcsfstimulation
bdutipo_str Repositorios
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