Efficient evaluation of a gene containment system for poplar through early flowering induction

Vertical gene transfer through pollen fow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene fow among tree species. However, evaluation of genetic containment strategies f...

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Detalles Bibliográficos
Autores principales: Briones, María Valentina, Hoenicka, Hans, Cañas, Luis A., Beltrán, José Pío, Hanelt, Dieter, Sharry, Sandra Elizabeth, Fladung, Matthias
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107399
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7165154&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107399
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
spellingShingle Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A.
Beltrán, José Pío
Hanelt, Dieter
Sharry, Sandra Elizabeth
Fladung, Matthias
Efficient evaluation of a gene containment system for poplar through early flowering induction
topic_facet Ciencias Agrarias
Botánica
Gene containment
Early fowering
Populus
Genetic transformation
PsEND1
Biosafety
description Vertical gene transfer through pollen fow from transgenic or non-native plant species into their crossable natural relatives is a major concern. Gene containment approaches have been proposed to reduce or even avoid gene fow among tree species. However, evaluation of genetic containment strategies for trees is very difcult due to the long-generation times. Early fowering induction would allow faster evaluation of genetic containment in this case. Although no reliable methods were available for the induction of fertile fowers in poplar, recently, a new early fowering approach was developed. In this study, early fowering poplar lines containing the gene construct PsEND1::barnase–barstar were obtained. The PsEND1 promoter was chosen due to its early expression pattern, its versality and efciency for generation of male-sterile plants fused to the barnase gene. RT-PCRs confrmed barnase gene activity in fowers, and pollen development was disturbed, leading to sterile fowers. The system developed in this study represents a valuable tool for gene containment studies in forest tree species.
format Articulo
Articulo
author Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A.
Beltrán, José Pío
Hanelt, Dieter
Sharry, Sandra Elizabeth
Fladung, Matthias
author_facet Briones, María Valentina
Hoenicka, Hans
Cañas, Luis A.
Beltrán, José Pío
Hanelt, Dieter
Sharry, Sandra Elizabeth
Fladung, Matthias
author_sort Briones, María Valentina
title Efficient evaluation of a gene containment system for poplar through early flowering induction
title_short Efficient evaluation of a gene containment system for poplar through early flowering induction
title_full Efficient evaluation of a gene containment system for poplar through early flowering induction
title_fullStr Efficient evaluation of a gene containment system for poplar through early flowering induction
title_full_unstemmed Efficient evaluation of a gene containment system for poplar through early flowering induction
title_sort efficient evaluation of a gene containment system for poplar through early flowering induction
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107399
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7165154&blobtype=pdf
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