Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina

The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-T...

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Autores principales: Schell, Celia María Beatriz, Tedim, Ana P., Rodríguez Baños, Mercedes, Sparo, Mónica Delfina, Lissarrague, Sabina, Basualdo Farjat, Juan Ángel, Coque, Teresa M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107346
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7168638&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107346
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
spellingShingle Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
Schell, Celia María Beatriz
Tedim, Ana P.
Rodríguez Baños, Mercedes
Sparo, Mónica Delfina
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Coque, Teresa M.
Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
topic_facet Ciencias Médicas
Enterococcus faecalis
Enterococcus faecium
invasive infections
antibiotic resistance
VRE
bla+
description The study’s aim was to analyze the population structure of enterococci causing human invasive infections in a medium-sized Argentinian Hospital coincidental with a 5 year-period of increased recovery of antibiotic resistant enterococci (2010–2014). Species identification (biochemical testing/MALDI-TOF-MS), antimicrobial susceptibility (disk-diffusion) and clonal relatedness (PFGE/MLST/BAPS) were determined according to standard guidelines. β-lactamase production was determined by a nitrocefin test and confirmed by PCR/sequencing. The isolates were identified as Enterococcus faecalis and Enterococcus faecium at a 2:1 ratio. Most of the E. faecalis isolates, grouped in 25 PFGE-types (ST9/ST179/ST236/ST281/ST388/ST604/ST720), were resistant to high-levels (HLR) of gentamicin/streptomycin. A ST9 clone (bla+/HLR-gentamicin) was detected in patients of different wards during 2014. E. faecium isolates were grouped in 10 PFGE-types (ST25/ST18/ST19/ST52/ST792), with a low rate of ampicillin resistance. Five vancomycin-resistant E. faecium, three vanA (ST792/ST25) and two vanB (ST25) were detected. The ST25 clone carried either vanA or vanB. The recovery of a bla+-ST9-E. faecalis clone similar to that described in the late 1980s in Argentina suggests the possibility of a local hidden reservoir. These results reflect the relevance of local epidemiology in understanding the population structure of enterococci as well as the emergence and spread of antimicrobial resistance in predominant enterococcal clonal lineages.
format Articulo
Articulo
author Schell, Celia María Beatriz
Tedim, Ana P.
Rodríguez Baños, Mercedes
Sparo, Mónica Delfina
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Coque, Teresa M.
author_facet Schell, Celia María Beatriz
Tedim, Ana P.
Rodríguez Baños, Mercedes
Sparo, Mónica Delfina
Lissarrague, Sabina
Basualdo Farjat, Juan Ángel
Coque, Teresa M.
author_sort Schell, Celia María Beatriz
title Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_short Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_full Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_fullStr Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_full_unstemmed Detection of β-Lactamase-Producing <i>Enterococcus faecalis</i> and Vancomycin-Resistant <i>Enterococcus faecium</i> Isolates in Human Invasive Infections in the Public Hospital of Tandil, Argentina
title_sort detection of β-lactamase-producing <i>enterococcus faecalis</i> and vancomycin-resistant <i>enterococcus faecium</i> isolates in human invasive infections in the public hospital of tandil, argentina
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/107346
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7168638&blobtype=pdf
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