Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice

Bacteria can be motile and planktonic or, alternatively, sessile and participating in the biofilm mode of growth. The transition between these lifestyles can be regulated by a second messenger, cyclic dimeric GMP (c-di-GMP). High intracellular c-diGMP concentration correlates with biofilm formation...

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Autores principales: Belhart, Keila, Gutierrez, María de la Paz, Zacca, Federico, Ambrosis, Nicolás, Cartelle Gestal, Monica, Taylor, Dawn, Dahlstrom, Kurt M., Harvill, Eric T., O'Toole, George A., Sisti, Federico, Fernández, Julieta
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Biología
Biofilm
Bordetella
Motility
c-di-GMP
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/107283
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6689298&blobtype=pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-107283
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biofilm
Bordetella
Motility
c-di-GMP
spellingShingle Biología
Biofilm
Bordetella
Motility
c-di-GMP
Belhart, Keila
Gutierrez, María de la Paz
Zacca, Federico
Ambrosis, Nicolás
Cartelle Gestal, Monica
Taylor, Dawn
Dahlstrom, Kurt M.
Harvill, Eric T.
O'Toole, George A.
Sisti, Federico
Fernández, Julieta
Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
topic_facet Biología
Biofilm
Bordetella
Motility
c-di-GMP
description Bacteria can be motile and planktonic or, alternatively, sessile and participating in the biofilm mode of growth. The transition between these lifestyles can be regulated by a second messenger, cyclic dimeric GMP (c-di-GMP). High intracellular c-diGMP concentration correlates with biofilm formation and motility inhibition in most bacteria, including Bordetella bronchiseptica, which causes respiratory tract infections in mammals and forms biofilms in infected mice. We previously described the diguanylate cyclase BdcA as involved in c-di-GMP synthesis and motility regulation in B. bronchiseptica; here, we further describe the mechanism whereby BdcA is able to regulate motility and biofilm formation. Amino acid replacement of GGDEF with GGAAF in BdcA is consistent with the conclusion that diguanylate cyclase activity is necessary for biofilm formation and motility regulation, although we were unable to confirm the stability of the mutant protein. In the absence of the bdcA gene, B. bronchiseptica showed enhanced motility, strengthening the hypothesis that BdcA regulates motility in B. bronchiseptica. We showed that c-di-GMP-mediated motility inhibition involved regulation of flagellin expression, as high c-di-GMP levels achieved by expressing BdcA significantly reduced the level of flagellin protein. We also demonstrated that protein BB2109 is necessary for BdcA activity, motility inhibition, and biofilm formation. Finally, absence of the bdcA gene affected bacterial infection, implicating BdcA-regulated functions as important for bacterium-host interactions. This work supports the role of c-di-GMP in biofilm formation and motility regulation in B. bronchiseptica, as well as its impact on pathogenesis.
format Articulo
Articulo
author Belhart, Keila
Gutierrez, María de la Paz
Zacca, Federico
Ambrosis, Nicolás
Cartelle Gestal, Monica
Taylor, Dawn
Dahlstrom, Kurt M.
Harvill, Eric T.
O'Toole, George A.
Sisti, Federico
Fernández, Julieta
author_facet Belhart, Keila
Gutierrez, María de la Paz
Zacca, Federico
Ambrosis, Nicolás
Cartelle Gestal, Monica
Taylor, Dawn
Dahlstrom, Kurt M.
Harvill, Eric T.
O'Toole, George A.
Sisti, Federico
Fernández, Julieta
author_sort Belhart, Keila
title Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
title_short Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
title_full Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
title_fullStr Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
title_full_unstemmed Bordetella bronchiseptica Diguanylate Cyclase BdcA Regulates Motility and Is Important for the Establishment of Respiratory Infection in Mice
title_sort bordetella bronchiseptica diguanylate cyclase bdca regulates motility and is important for the establishment of respiratory infection in mice
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/107283
http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6689298&blobtype=pdf
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