Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2
Microbe-host interactions are generally homeostatic, but when dysfunctional, they can incite food sensitivities and chronic diseases. Celiac disease (CeD) is a food sensitivity characterized by a breakdown of oral tolerance to gluten proteins in genetically predisposed individuals, although the unde...
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| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/107005 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6416356&blobtype=pdf |
| Aporte de: |
| id |
I19-R120-10915-107005 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Proteases Microbe-host interactions Food sensitivities |
| spellingShingle |
Biología Proteases Microbe-host interactions Food sensitivities Caminero, Alberto McCarville, Justin L. Galipeau, Heather J. Deraison, Celine Bernier, Steve P. Constante, Marco Rolland, Corinne Meisel, Marlies Murray, Joseph A. Yu, Xuechen B. Alaedini, Armin Coombes, Brian K. Bercik, Premysl Southward, Carolyn M. Ruf, Wolfram Jabri, Bana Chirdo, Fernando Gabriel Casqueiro, Javier Surette, Michael G. Vergnolle, Nathalie Verdu, Elena F. Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| topic_facet |
Biología Proteases Microbe-host interactions Food sensitivities |
| description |
Microbe-host interactions are generally homeostatic, but when dysfunctional, they can incite food sensitivities and chronic diseases. Celiac disease (CeD) is a food sensitivity characterized by a breakdown of oral tolerance to gluten proteins in genetically predisposed individuals, although the underlying mechanisms are incompletely understood. Here we show that duodenal biopsies from patients with active CeD have increased proteolytic activity against gluten substrates that correlates with increased Proteobacteria abundance, including Pseudomonas. Using Pseudomonas aeruginosa producing elastase as a model, we show gluten-independent, PAR-2 mediated upregulation of inflammatory pathways in C57BL/6 mice without villus blunting. In mice expressing CeD risk genes, P. aeruginosa elastase synergizes with gluten to induce more severe inflammation that is associated with moderate villus blunting. These results demonstrate that proteases expressed by opportunistic pathogens impact host immune responses that are relevant to the development of food sensitivities, independently of the trigger antigen. |
| format |
Articulo Articulo |
| author |
Caminero, Alberto McCarville, Justin L. Galipeau, Heather J. Deraison, Celine Bernier, Steve P. Constante, Marco Rolland, Corinne Meisel, Marlies Murray, Joseph A. Yu, Xuechen B. Alaedini, Armin Coombes, Brian K. Bercik, Premysl Southward, Carolyn M. Ruf, Wolfram Jabri, Bana Chirdo, Fernando Gabriel Casqueiro, Javier Surette, Michael G. Vergnolle, Nathalie Verdu, Elena F. |
| author_facet |
Caminero, Alberto McCarville, Justin L. Galipeau, Heather J. Deraison, Celine Bernier, Steve P. Constante, Marco Rolland, Corinne Meisel, Marlies Murray, Joseph A. Yu, Xuechen B. Alaedini, Armin Coombes, Brian K. Bercik, Premysl Southward, Carolyn M. Ruf, Wolfram Jabri, Bana Chirdo, Fernando Gabriel Casqueiro, Javier Surette, Michael G. Vergnolle, Nathalie Verdu, Elena F. |
| author_sort |
Caminero, Alberto |
| title |
Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| title_short |
Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| title_full |
Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| title_fullStr |
Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| title_full_unstemmed |
Duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| title_sort |
duodenal bacterial proteolytic activity determines sensitivity to dietary antigen through protease-activated receptor-2 |
| publishDate |
2019 |
| url |
http://sedici.unlp.edu.ar/handle/10915/107005 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC6416356&blobtype=pdf |
| work_keys_str_mv |
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Repositorios |
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