High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice

Ventricular arrhythmias are a common cause of sudden cardiac death, and their occurrence is higher in obese subjects. Abnormal gating of ryanodine receptors (RyR2), the calcium release channels of the sarcoplasmic reticulum, can produce ventricular arrhythmias. Since obesity promotes oxidative stres...

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Autores principales:	Sánchez, Gina, Araneda, Felipe, Peña, Juan Pedro, Finkelstein, José Pablo, Riquelme, Jaime A., Montecinos, Luis, Barrientos, Genaro, Llanos, Paola, Pedrozo, Zully, Said, María Matilde, Bull, Ricardo, Donoso, Paulina
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2018
Materias:	Ciencias Médicas calcium release channels reactive oxygen species (ROS) redox modifications ventricular tachycardia NADPH oxidase
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/106805 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5855755&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-106805
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas calcium release channels reactive oxygen species (ROS) redox modifications ventricular tachycardia NADPH oxidase
spellingShingle	Ciencias Médicas calcium release channels reactive oxygen species (ROS) redox modifications ventricular tachycardia NADPH oxidase Sánchez, Gina Araneda, Felipe Peña, Juan Pedro Finkelstein, José Pablo Riquelme, Jaime A. Montecinos, Luis Barrientos, Genaro Llanos, Paola Pedrozo, Zully Said, María Matilde Bull, Ricardo Donoso, Paulina High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice
topic_facet	Ciencias Médicas calcium release channels reactive oxygen species (ROS) redox modifications ventricular tachycardia NADPH oxidase
description	Ventricular arrhythmias are a common cause of sudden cardiac death, and their occurrence is higher in obese subjects. Abnormal gating of ryanodine receptors (RyR2), the calcium release channels of the sarcoplasmic reticulum, can produce ventricular arrhythmias. Since obesity promotes oxidative stress and RyR2 are redox-sensitive channels, we investigated whether the RyR2 activity was altered in obese mice. Mice fed a high fat diet (HFD) became obese after eight weeks and exhibited a significant increase in the occurrence of ventricular arrhythmias. Single RyR2 channels isolated from the hearts of obese mice were more active in planar bilayers than those isolated from the hearts of the control mice. At the molecular level, RyR2 channels from HFD-fed mice had substantially fewer free thiol residues, suggesting that redox modifications were responsible for the higher activity. Apocynin, provided in the drinking water, completely prevented the appearance of ventricular arrhythmias in HFD-fed mice, and normalized the activity and content of the free thiol residues of the protein. HFD increased the expression of NOX4, an isoform of NADPH oxidase, in the heart. Our results suggest that HFD increases the activity of RyR2 channels via a redox-dependent mechanism, favoring the appearance of ventricular arrhythmias.
format	Articulo Articulo
author	Sánchez, Gina Araneda, Felipe Peña, Juan Pedro Finkelstein, José Pablo Riquelme, Jaime A. Montecinos, Luis Barrientos, Genaro Llanos, Paola Pedrozo, Zully Said, María Matilde Bull, Ricardo Donoso, Paulina
author_facet	Sánchez, Gina Araneda, Felipe Peña, Juan Pedro Finkelstein, José Pablo Riquelme, Jaime A. Montecinos, Luis Barrientos, Genaro Llanos, Paola Pedrozo, Zully Said, María Matilde Bull, Ricardo Donoso, Paulina
author_sort	Sánchez, Gina
title	High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice
title_short	High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice
title_full	High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice
title_fullStr	High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice
title_full_unstemmed	High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice
title_sort	high-fat-diet-induced obesity produces spontaneous ventricular arrhythmias and increases the activity of ryanodine receptors in mice
publishDate	2018
url	http://sedici.unlp.edu.ar/handle/10915/106805 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5855755&blobtype=pdf
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High-Fat-Diet-Induced Obesity Produces Spontaneous Ventricular Arrhythmias and Increases the Activity of Ryanodine Receptors in Mice

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