Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells

Ionotropic purinergic receptors (P2X) are expressed in endothelial and smooth muscle cells of blood vessels. ATP acting on smooth muscle P2X receptors is able to induce vasoconstriction in different kind of vessels. However, to our knowledge, there are no reports that directly show the activity of t...

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Autores principales: Enrique, Nicolás Jorge, Rebolledo, Alejandro, Martín, Pedro, Roldán Palomo, Ana Rocío, Tanzi, F., Milesi, María Verónica
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Médicas
ATP
Human umbilical artery
Patch clamp
Microfluorimetry
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/106759
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-106759
record_format dspace
spelling I19-R120-10915-1067592024-06-14T18:48:38Z http://sedici.unlp.edu.ar/handle/10915/106759 Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells Enrique, Nicolás Jorge Rebolledo, Alejandro Martín, Pedro Roldán Palomo, Ana Rocío Tanzi, F. Milesi, María Verónica 2012 2020-10-13T12:47:04Z en Ciencias Médicas ATP Human umbilical artery Patch clamp Microfluorimetry Ionotropic purinergic receptors (P2X) are expressed in endothelial and smooth muscle cells of blood vessels. ATP acting on smooth muscle P2X receptors is able to induce vasoconstriction in different kind of vessels. However, to our knowledge, there are no reports that directly show the activity of these purinergic receptors in native human vascular smooth muscle cells. In this work, we describe for the first time an ATP-induced current in freshly isolated human umbilical artery (HUA) smooth muscle cells. The current was measured by patch-clamp technique in whole-cell condition on cells clamped at −50 mV. At 100 μM of ATP the current showed a rapid activation and desensitization, and was carried by both Na+ and Ca2+. The current was completely blocked by suramin (300 μM) and partially blocked by 100 μM of Zn2+ without affecting the kinetic of desensitization. All these properties suggest that the ATP-induced ionic currents are mediated through P2X1-like receptors. Moreover, we show that ATP transiently increased cytosolic Ca2+ in “in situ” smooth muscle cells of intact HUA segments and that this response is dependent of extracellular and intracellular Ca2+. These data expand the knowledge of purinergic receptors properties in vascular smooth muscle cells and the probable role of ATP as a paracrine modulator of contractile tone in a human artery which is fundamental for feto-placental blood flow. Facultad de Ciencias Exactas Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 199-206
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
ATP
Human umbilical artery
Patch clamp
Microfluorimetry
spellingShingle Ciencias Médicas
ATP
Human umbilical artery
Patch clamp
Microfluorimetry
Enrique, Nicolás Jorge
Rebolledo, Alejandro
Martín, Pedro
Roldán Palomo, Ana Rocío
Tanzi, F.
Milesi, María Verónica
Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells
topic_facet Ciencias Médicas
ATP
Human umbilical artery
Patch clamp
Microfluorimetry
description Ionotropic purinergic receptors (P2X) are expressed in endothelial and smooth muscle cells of blood vessels. ATP acting on smooth muscle P2X receptors is able to induce vasoconstriction in different kind of vessels. However, to our knowledge, there are no reports that directly show the activity of these purinergic receptors in native human vascular smooth muscle cells. In this work, we describe for the first time an ATP-induced current in freshly isolated human umbilical artery (HUA) smooth muscle cells. The current was measured by patch-clamp technique in whole-cell condition on cells clamped at −50 mV. At 100 μM of ATP the current showed a rapid activation and desensitization, and was carried by both Na+ and Ca2+. The current was completely blocked by suramin (300 μM) and partially blocked by 100 μM of Zn2+ without affecting the kinetic of desensitization. All these properties suggest that the ATP-induced ionic currents are mediated through P2X1-like receptors. Moreover, we show that ATP transiently increased cytosolic Ca2+ in “in situ” smooth muscle cells of intact HUA segments and that this response is dependent of extracellular and intracellular Ca2+. These data expand the knowledge of purinergic receptors properties in vascular smooth muscle cells and the probable role of ATP as a paracrine modulator of contractile tone in a human artery which is fundamental for feto-placental blood flow.
format Articulo
Articulo
author Enrique, Nicolás Jorge
Rebolledo, Alejandro
Martín, Pedro
Roldán Palomo, Ana Rocío
Tanzi, F.
Milesi, María Verónica
author_facet Enrique, Nicolás Jorge
Rebolledo, Alejandro
Martín, Pedro
Roldán Palomo, Ana Rocío
Tanzi, F.
Milesi, María Verónica
author_sort Enrique, Nicolás Jorge
title Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells
title_short Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells
title_full Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells
title_fullStr Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells
title_full_unstemmed Extracellular ATP induces fast and transient non-selective cationic currents and cytosolic Ca2+ changes in human umbilical artery smooth muscle cells
title_sort extracellular atp induces fast and transient non-selective cationic currents and cytosolic ca2+ changes in human umbilical artery smooth muscle cells
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/106759
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AT rebolledoalejandro extracellularatpinducesfastandtransientnonselectivecationiccurrentsandcytosolicca2changesinhumanumbilicalarterysmoothmusclecells
AT martinpedro extracellularatpinducesfastandtransientnonselectivecationiccurrentsandcytosolicca2changesinhumanumbilicalarterysmoothmusclecells
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AT tanzif extracellularatpinducesfastandtransientnonselectivecationiccurrentsandcytosolicca2changesinhumanumbilicalarterysmoothmusclecells
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