Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration
Diaphragmatic myoblasts (DMs) are precursors of type-1 muscle cells displaying high exhaustion threshold on account that they contract and relax 20 times/min over a lifespan, making them potentially useful in cardiac regeneration strategies. Besides, it has been shown that biomaterials for stem cell...
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| Autores principales: | , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/106493 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5851959&blobtype=pdf |
| Aporte de: |
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I19-R120-10915-106493 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Diaphragm Myoblasts Connexin-43 Absorbable implants Poly (L-lactic acid) Sheep Fluorescence recovery after photobleaching |
| spellingShingle |
Ciencias Médicas Diaphragm Myoblasts Connexin-43 Absorbable implants Poly (L-lactic acid) Sheep Fluorescence recovery after photobleaching Giménez, Carlos Sebastián Locatelli, Paola Montini Ballarin, Florencia Orlowski, Alejandro Dewey, Ricardo Alfredo Pena, Milagros Abraham, Gustavo Abel Aiello, Ernesto Alejandro Bauzá, María del Rosario Cuniberti, Luis Olea, Fernanda Daniela Crottogini, Alberto Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration |
| topic_facet |
Ciencias Médicas Diaphragm Myoblasts Connexin-43 Absorbable implants Poly (L-lactic acid) Sheep Fluorescence recovery after photobleaching |
| description |
Diaphragmatic myoblasts (DMs) are precursors of type-1 muscle cells displaying high exhaustion threshold on account that they contract and relax 20 times/min over a lifespan, making them potentially useful in cardiac regeneration strategies. Besides, it has been shown that biomaterials for stem cell delivery improve cell retention and viability in the target organ. In the present study, we aimed at developing a novel approach based on the use of poly (L-lactic acid) (PLLA) scaffolds seeded with DMs overexpressing connexin-43 (cx43), a gap junction protein that promotes inter-cell connectivity. DMs isolated from ovine diaphragm biopsies were characterized by immunohistochemistry and ability to differentiate into myotubes (MTs) and transduced with a lentiviral vector encoding cx43. After confirming cx43 expression (RT-qPCR and Western blot) and its effect on inter-cell connectivity (fluorescence recovery after photobleaching), DMs were grown on fiberaligned or random PLLA scaffolds. DMs were successfully isolated and characterized. Cx43 mRNA and protein were overexpressed and favored inter-cell connectivity. Alignment of the scaffold fibers not only aligned but also elongated the cells, increasing the contact surface between them. This novel approach is feasible and combines the advantages of bioresorbable scaffolds as delivery method and a cell type that on account of its features may be suitable for cardiac regeneration. Future studies on animal models of myocardial infarction are needed to establish its usefulness on scar reduction and cardiac function. |
| format |
Articulo Articulo |
| author |
Giménez, Carlos Sebastián Locatelli, Paola Montini Ballarin, Florencia Orlowski, Alejandro Dewey, Ricardo Alfredo Pena, Milagros Abraham, Gustavo Abel Aiello, Ernesto Alejandro Bauzá, María del Rosario Cuniberti, Luis Olea, Fernanda Daniela Crottogini, Alberto |
| author_facet |
Giménez, Carlos Sebastián Locatelli, Paola Montini Ballarin, Florencia Orlowski, Alejandro Dewey, Ricardo Alfredo Pena, Milagros Abraham, Gustavo Abel Aiello, Ernesto Alejandro Bauzá, María del Rosario Cuniberti, Luis Olea, Fernanda Daniela Crottogini, Alberto |
| author_sort |
Giménez, Carlos Sebastián |
| title |
Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration |
| title_short |
Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration |
| title_full |
Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration |
| title_fullStr |
Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration |
| title_full_unstemmed |
Aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (L-lactic acid) scaffolds for potential use in cardiac regeneration |
| title_sort |
aligned ovine diaphragmatic myoblasts overexpressing human connexin-43 seeded on poly (l-lactic acid) scaffolds for potential use in cardiac regeneration |
| publishDate |
2018 |
| url |
http://sedici.unlp.edu.ar/handle/10915/106493 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5851959&blobtype=pdf |
| work_keys_str_mv |
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Repositorios |
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1764820442656276481 |