Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles

Intestinal and liver fatty acid binding proteins (IFABP and LFABP, respectively), are cytosolic soluble proteins with the capacity to bind and transport hydrophobic ligands between different sub-cellular compartments. Their functions are still not clear but they are supposed to be involved in lipid...

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Detalles Bibliográficos
Autores principales:	Falomir Lockhart, Lisandro Jorge, Franchini, Gisela Raquel, Guerbi, María Ximena, Storch, Judith, Córsico, Betina
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2011
Materias:	Ciencias Médicas Fatty Acid Binding Proteins Membrane Interaction Intracellular Fatty Acid Traffic Model Membranes
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/106489 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3143005&blobtype=pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-106489
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Fatty Acid Binding Proteins Membrane Interaction Intracellular Fatty Acid Traffic Model Membranes
spellingShingle	Ciencias Médicas Fatty Acid Binding Proteins Membrane Interaction Intracellular Fatty Acid Traffic Model Membranes Falomir Lockhart, Lisandro Jorge Franchini, Gisela Raquel Guerbi, María Ximena Storch, Judith Córsico, Betina Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles
topic_facet	Ciencias Médicas Fatty Acid Binding Proteins Membrane Interaction Intracellular Fatty Acid Traffic Model Membranes
description	Intestinal and liver fatty acid binding proteins (IFABP and LFABP, respectively), are cytosolic soluble proteins with the capacity to bind and transport hydrophobic ligands between different sub-cellular compartments. Their functions are still not clear but they are supposed to be involved in lipid trafficking and metabolism, cell growth, and regulation of several other processes, like cell differentiation. Here we investigated the interaction of these proteins with different models of phospholipid membrane vesicles in order to achieve further insight into their specificity within the enterocyte. A combination of biophysical and biochemical techniques allowed us to determine affinities of these proteins to membranes, the way phospholipid composition and vesicle size and curvature modulate such interaction, as well as the effect of protein binding on the integrity of the membrane structure. We demonstrate here that, beside their apparently opposite ligand transfer mechanisms, both LFABP and IFABP are able to interact with phospholipid membranes, but the factors that modulate such interactions are different for each protein, further implying different roles for IFABP and LFABP in the intracellular context. These results contribute to the proposed central role of intestinal FABPs in the lipid traffic within enterocytes as well as in the regulation of more complex cellular processes.
format	Articulo Preprint
author	Falomir Lockhart, Lisandro Jorge Franchini, Gisela Raquel Guerbi, María Ximena Storch, Judith Córsico, Betina
author_facet	Falomir Lockhart, Lisandro Jorge Franchini, Gisela Raquel Guerbi, María Ximena Storch, Judith Córsico, Betina
author_sort	Falomir Lockhart, Lisandro Jorge
title	Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles
title_short	Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles
title_full	Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles
title_fullStr	Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles
title_full_unstemmed	Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles
title_sort	interaction of enterocyte fabps with phospholipid membranes: clues for specific physiological roles
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/106489 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC3143005&blobtype=pdf
work_keys_str_mv	AT falomirlockhartlisandrojorge interactionofenterocytefabpswithphospholipidmembranescluesforspecificphysiologicalroles AT franchinigiselaraquel interactionofenterocytefabpswithphospholipidmembranescluesforspecificphysiologicalroles AT guerbimariaximena interactionofenterocytefabpswithphospholipidmembranescluesforspecificphysiologicalroles AT storchjudith interactionofenterocytefabpswithphospholipidmembranescluesforspecificphysiologicalroles AT corsicobetina interactionofenterocytefabpswithphospholipidmembranescluesforspecificphysiologicalroles
bdutipo_str	Repositorios
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Interaction of enterocyte FABPs with phospholipid membranes: clues for specific physiological roles

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