Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>

This investigation provides evidence of the acyl enzyme species involved in the interaction of <i>R/S</i>ketoprofen with the lipase B from <i>Candida antarctica</i>. The interaction between the profen and the enzyme was studied by <i>in situ</i> time-resolved ATR-...

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Autores principales: Toledo, María Victoria, Llerena Suster, Carlos Rafael, Ferreira, María L., Collins, Sebastián E., Briand, Laura Estefanía
Formato: Articulo
Lenguaje:Español
Publicado: 2017
Materias:
Ciencias Exactas
Candida antarctica
acyl enzyme species
ketoprofen
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/105708
https://pubs.rsc.org/en/content/articlelanding/2017/CY/C7CY00245A
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-105708
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Exactas
Candida antarctica
acyl enzyme species
ketoprofen
spellingShingle Ciencias Exactas
Candida antarctica
acyl enzyme species
ketoprofen
Toledo, María Victoria
Llerena Suster, Carlos Rafael
Ferreira, María L.
Collins, Sebastián E.
Briand, Laura Estefanía
Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>
topic_facet Ciencias Exactas
Candida antarctica
acyl enzyme species
ketoprofen
description This investigation provides evidence of the acyl enzyme species involved in the interaction of <i>R/S</i>ketoprofen with the lipase B from <i>Candida antarctica</i>. The interaction between the profen and the enzyme was studied by <i>in situ</i> time-resolved ATR-FTIR under both static and transient conditions. Particularly, modulation excitation spectroscopy (MES) with phase sensitive detection (PSD) allowed us to univocally distinguish the signals belonging to the interaction between ketoprofen and the enzyme from the strong background signals. These experimental tools coupled with theoretical DFT analysis allowed us to propose various species derived from the interaction of ketoprofen with serine through H bonding (without reaction) and the acyl enzyme species (ester bond formation) which are the intermediates in the biocatalytic assisted esterification and hydrolysis using lipases.
format Articulo
Articulo
author Toledo, María Victoria
Llerena Suster, Carlos Rafael
Ferreira, María L.
Collins, Sebastián E.
Briand, Laura Estefanía
author_facet Toledo, María Victoria
Llerena Suster, Carlos Rafael
Ferreira, María L.
Collins, Sebastián E.
Briand, Laura Estefanía
author_sort Toledo, María Victoria
title Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>
title_short Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>
title_full Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>
title_fullStr Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>
title_full_unstemmed Molecular recognition of an acyl–enzyme intermediate on the lipase B from <i>Candida antarctica</i>
title_sort molecular recognition of an acyl–enzyme intermediate on the lipase b from <i>candida antarctica</i>
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/105708
https://pubs.rsc.org/en/content/articlelanding/2017/CY/C7CY00245A
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AT ferreiramarial molecularrecognitionofanacylenzymeintermediateonthelipasebfromicandidaantarcticai
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