Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells
The aim of this work was to evaluate the ability of a kefir-isolated microbial mixture containing three bacterial and two yeast strains (MM) to protect intestinal epithelial cells against <i>Shigella flexneri</i> invasion, as well as to analyse the effect on pro-inflammatory response eli...
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2016
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/104974 https://www.wageningenacademic.com/doi/10.3920/BM2015.0061 |
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I19-R120-10915-104974 |
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Universidad Nacional de La Plata |
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I-19 |
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R-120 |
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SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Exactas Química Biología kefir microorganisms Shigella flexneri pro-inflammatory response |
spellingShingle |
Ciencias Exactas Química Biología kefir microorganisms Shigella flexneri pro-inflammatory response Bolla, Patricia Araceli Abraham, Analía Graciela Pérez, Pablo Fernando Serradell, María de los Ángeles Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
topic_facet |
Ciencias Exactas Química Biología kefir microorganisms Shigella flexneri pro-inflammatory response |
description |
The aim of this work was to evaluate the ability of a kefir-isolated microbial mixture containing three bacterial and two yeast strains (MM) to protect intestinal epithelial cells against <i>Shigella flexneri</i> invasion, as well as to analyse the effect on pro-inflammatory response elicited by this pathogen. A significant decrease in <i>S. flexneri</i> strain 72 invasion was observed on both HT-29 and Caco-2 cells pre-incubated with MM. Pre-incubation with the individual strains <i>Saccharomyces cerevisiae</i> CIDCA 8112 or <i>Lactococcus lactis</i> subsp. <i>lactis</i> CIDCA 8221 also reduced the internalisation of <i>S. flexneri</i> into HT-29 cells although in a lesser extent than MM. Interestingly, <i>Lactobacillus plantarum</i> CIDCA 83114 exerted a protective effect on the invasion of Caco-2 and HT-29 cells by <i>S. flexneri</i>. Regarding the pro-inflammatory response on HT-29 cells, <i>S. flexneri</i> infection induced a significant activation of the expression of interleukin 8 (IL-8), chemokine (C-C motif) ligand 20 (CCL20) and tumour necrosis factor alpha (TNF-α) encoding genes (P<0.05), whereas incubation of cells with MM did not induce the expression of any of the mediators assessed. Interestingly, pre-incubation of HT-29 monolayer with MM produced an inhibition of <i>S. flexneri</i>-induced IL-8, CCL20 and TNF-α mRNA expression. In order to gain insight on the effect of MM (or the individual strains) on this pro-inflammatory response, a series of experiments using a HT-29-NF-κB-hrGFP reporter system were performed. Pre-incubation of HT-29-NF-κB-hrGFP cells with MM significantly dampened Shigella-induced activation. Our results showed that the contribution of yeast strain <i>Kluyveromyces marxianus</i> CIDCA 8154 seems to be crucial in the observed effect. In conclusion, results presented in this study demonstrate that pre-treatment with a microbial mixture containing bacteria and yeasts isolated from kefir, resulted in inhibition of <i>S. flexneri</i> internalisation into human intestinal epithelial cells, along with the inhibition of the signalling via NF-κB that in turn led to the attenuation of the inflammatory response. |
format |
Articulo Articulo |
author |
Bolla, Patricia Araceli Abraham, Analía Graciela Pérez, Pablo Fernando Serradell, María de los Ángeles |
author_facet |
Bolla, Patricia Araceli Abraham, Analía Graciela Pérez, Pablo Fernando Serradell, María de los Ángeles |
author_sort |
Bolla, Patricia Araceli |
title |
Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
title_short |
Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
title_full |
Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
title_fullStr |
Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
title_full_unstemmed |
Kefir-isolated bacteria and yeasts inhibit <i>Shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
title_sort |
kefir-isolated bacteria and yeasts inhibit <i>shigella flexneri</i> invasion and modulate pro-inflammatory response on intestinal epithelial cells |
publishDate |
2016 |
url |
http://sedici.unlp.edu.ar/handle/10915/104974 https://www.wageningenacademic.com/doi/10.3920/BM2015.0061 |
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Repositorios |
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