The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development

The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturati...

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Detalles Bibliográficos
Autores principales: Sirini, Matías Ángel, Anchordoquy, Juan Mateo, Anchordoquy, Juan Patricio, Pascua, Ana Malen, Nikoloff, Noelia, Carranza Martín, Ana Cristina, Relling, Alejandro Enrique, Furnus, Cecilia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Veterinarias
Zoología
Active ghrelin
Apoptosis
Cumulus cells
DNA damage
In vitro embryo development
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104868
Aporte de:
Aportado por : SEDICI (UNLP) de Universidad Nacional de La Plata .
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spelling I19-R120-10915-1048682020-10-26T04:06:38Z http://sedici.unlp.edu.ar/handle/10915/104868 issn:1469-8730 The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development Sirini, Matías Ángel Anchordoquy, Juan Mateo Anchordoquy, Juan Patricio Pascua, Ana Malen Nikoloff, Noelia Carranza Martín, Ana Cristina Relling, Alejandro Enrique Furnus, Cecilia Cristina 2017-10 2020-09-17T15:16:45Z en Ciencias Veterinarias Zoología Active ghrelin Apoptosis Cumulus cells DNA damage In vitro embryo development The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturation parameters. Cumulus–oocyte complexes (COC) were assessed with the comet, apoptosis and viability assays. The in vitro effects of acylated ghrelin on embryo developmental capacity and embryo quality were also evaluated. Results demonstrated that acylated ghrelin did not affect oocyte nuclear maturation and cumulus expansion area. However, it induced cumulus cell (CC) death, apoptosis and DNA damage. The damage increased as a function of the concentration employed. Additionally, the percentages of blastocyst yield, hatching and embryo quality decreased with all acylated ghrelin concentrations tested. Our study highlights the importance of acylated ghrelin in bovine reproduction, suggesting that this metabolic hormone could function as a signal that prevents the progress to reproductive processes. Instituto de Genética Veterinaria Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 601-611
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Zoología
Active ghrelin
Apoptosis
Cumulus cells
DNA damage
In vitro embryo development
spellingShingle Ciencias Veterinarias
Zoología
Active ghrelin
Apoptosis
Cumulus cells
DNA damage
In vitro embryo development
Sirini, Matías Ángel
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Pascua, Ana Malen
Nikoloff, Noelia
Carranza Martín, Ana Cristina
Relling, Alejandro Enrique
Furnus, Cecilia Cristina
The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
topic_facet Ciencias Veterinarias
Zoología
Active ghrelin
Apoptosis
Cumulus cells
DNA damage
In vitro embryo development
description The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturation parameters. Cumulus–oocyte complexes (COC) were assessed with the comet, apoptosis and viability assays. The in vitro effects of acylated ghrelin on embryo developmental capacity and embryo quality were also evaluated. Results demonstrated that acylated ghrelin did not affect oocyte nuclear maturation and cumulus expansion area. However, it induced cumulus cell (CC) death, apoptosis and DNA damage. The damage increased as a function of the concentration employed. Additionally, the percentages of blastocyst yield, hatching and embryo quality decreased with all acylated ghrelin concentrations tested. Our study highlights the importance of acylated ghrelin in bovine reproduction, suggesting that this metabolic hormone could function as a signal that prevents the progress to reproductive processes.
format Articulo
Articulo
author Sirini, Matías Ángel
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Pascua, Ana Malen
Nikoloff, Noelia
Carranza Martín, Ana Cristina
Relling, Alejandro Enrique
Furnus, Cecilia Cristina
author_facet Sirini, Matías Ángel
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Pascua, Ana Malen
Nikoloff, Noelia
Carranza Martín, Ana Cristina
Relling, Alejandro Enrique
Furnus, Cecilia Cristina
author_sort Sirini, Matías Ángel
title The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
title_short The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
title_full The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
title_fullStr The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
title_full_unstemmed The presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell DNA damage and apoptosis, and impairs early embryo development
title_sort presence of acylated ghrelin during in vitro maturation of bovine oocytes induces cumulus cell dna damage and apoptosis, and impairs early embryo development
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/104868
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