Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography

In reversed-phase liquid chromatography (RPLC), the retention of weak acids and bases is a sigmoidal function of the mobile-phase pH. Therefore, pH is a key chromatographic variable to optimize retention and selectivity. Furthermore, at an eluent pH close to the pKa of the solute, the dependence of...

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Autores principales: Gagliardi, Leonardo Gabriel, Castells, Cecilia Beatriz Marta, Ràfols, Clara, Rosés, Martí, Bosch, Elisabeth
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Química
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104803
http://hdl.handle.net/11336/95500
https://pubs.acs.org/doi/abs/10.1021/ac060482i
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104803
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
spellingShingle Química
Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography
topic_facet Química
description In reversed-phase liquid chromatography (RPLC), the retention of weak acids and bases is a sigmoidal function of the mobile-phase pH. Therefore, pH is a key chromatographic variable to optimize retention and selectivity. Furthermore, at an eluent pH close to the pKa of the solute, the dependence of ionization of the buffer and solute on temperature can be used to improve chromatographic separations involving ionizable solutes by an adequate handling of column temperature. In this paper, we derive a general equation for the prediction of the retentive behavior of ionizable compounds upon simultaneous changes in mobile-phase pH and column temperature. Four experiments, two limiting pH values and two temperatures, provide the input data that allow predictions in the whole range of these two variables, based on the thermodynamic fundamentals of the involved equilibria. Also, the study demonstrates the significant role that the choice of the buffer compound would have on selectivity factors in RPLC at temperatures higher than 25 °C.
format Articulo
Articulo
author Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author_facet Gagliardi, Leonardo Gabriel
Castells, Cecilia Beatriz Marta
Ràfols, Clara
Rosés, Martí
Bosch, Elisabeth
author_sort Gagliardi, Leonardo Gabriel
title Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography
title_short Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography
title_full Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography
title_fullStr Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography
title_full_unstemmed Modeling retention and selectivity as a function of pH and column temperature in liquid chromatography
title_sort modeling retention and selectivity as a function of ph and column temperature in liquid chromatography
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/104803
http://hdl.handle.net/11336/95500
https://pubs.acs.org/doi/abs/10.1021/ac060482i
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