Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis

The purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis- infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 co...

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Autores principales: Guedes, Roberto M. C., Machuca, Mariana Alejandra, Quiroga, María Alejandra, Real Pereira, Carlos E., Resende, Talita P., Gebhart, Connie J.
Formato: Articulo
Lenguaje:Español
Publicado: 2017
Materias:
Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104798
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104798
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
spellingShingle Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
Guedes, Roberto M. C.
Machuca, Mariana Alejandra
Quiroga, María Alejandra
Real Pereira, Carlos E.
Resende, Talita P.
Gebhart, Connie J.
Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
topic_facet Ciencias Veterinarias
Apoptosis
Caspase-3
Immunohistochemistry
Lawsonia intracellularis
Pathogenesis
Proliferative enteropathy
Swine
TUNEL
description The purpose of this study was to follow the progression of gross and histologic lesions and apoptosis events in Lawsonia intracellularis- infected enterocytes through the course of the disease, proliferative enteropathy (PE). Thirty 5-week-old pigs were divided into 2 groups: 20 challenged and 10 control animals. Groups of 3 pigs, 2 challenged and 1 control, were euthanized at 1, 3, 5, 8, 11, 15, 19, 24, 29, and 35 days after inoculation. Complete necropsies were performed with gross evaluation. Tissue samples from different sites of the gastrointestinal tract and other visceral organs were collected for routine histologic staining and for immunohistochemistry (IHC) for L. intracellularis. In addition, caspase-3, terminal deoxyuridine nick-end labeling assay, and electron microscopy were performed in ileum samples. Macroscopic and histologic lesions suggestive of PE were first detected 11 days after infection and continued through day 24. L. intracellularis antigen was first detected in the intestine by IHC on day 5 after inoculation, and the bacterium was first detected by transmission electron microscopy on day 15. Positive IHC staining for [L. intracellularis] and enterocyte proliferation, but no gross lesion, were detected on day 29. All 3 pigs euthanized on day 35 were grossly and histologically normal and IHC negative. Hyperplastic crypts in challenge pigs had more apoptotic cells on days 15, 19, and 24 postinfection (P < .05) compared to control pigs. Our results demonstrated the progression of lesions and infection by L. intracellularis and that inhibition of enterocyte apoptosis is not involved in the pathogenesis of proliferative enteropathy.
format Articulo
Articulo
author Guedes, Roberto M. C.
Machuca, Mariana Alejandra
Quiroga, María Alejandra
Real Pereira, Carlos E.
Resende, Talita P.
Gebhart, Connie J.
author_facet Guedes, Roberto M. C.
Machuca, Mariana Alejandra
Quiroga, María Alejandra
Real Pereira, Carlos E.
Resende, Talita P.
Gebhart, Connie J.
author_sort Guedes, Roberto M. C.
title Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_short Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_full Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_fullStr Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_full_unstemmed Lawsonia intracellularis in pigs: progression of lesions and Involvement of apoptosis
title_sort lawsonia intracellularis in pigs: progression of lesions and involvement of apoptosis
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/104798
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