Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness
<i>Fusarium graminearum</i> is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination. The aim of this work was to analyze the phenotypic characterist...
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| Autores principales: | , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/104752 |
| Aporte de: |
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I19-R120-10915-104752 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Ciencias Agrarias Fusarium graminearum FTIR spectroscopy Phenotypic diversity Aggressiveness |
| spellingShingle |
Biología Ciencias Agrarias Fusarium graminearum FTIR spectroscopy Phenotypic diversity Aggressiveness Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, María Alejandra Alconada Magliano, Teresa María Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| topic_facet |
Biología Ciencias Agrarias Fusarium graminearum FTIR spectroscopy Phenotypic diversity Aggressiveness |
| description |
<i>Fusarium graminearum</i> is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects crop yields and grain quality also reducing the wheat end-use, and causing mycotoxin contamination.
The aim of this work was to analyze the phenotypic characteristics associated with phenotypic diversity and aggressiveness of 34 <i>F. graminearum</i> <i>sensu stricto</i> isolates recovered from Argentinean fields in the 2008 growing season using the Fourier Transform Infrared (FTIR) dried film technology. We applied this technique also to search for spectral specific markers associatedwith aggressiveness. The combination of FTIR technologywith hierarchical cluster analysis allowed us to determine that this population constitutes a highly diverse and heterogeneous group of fungi with significant phenotypic variance. Still, when the spectral features of a set of these isolates were compared against their aggressiveness, as measured by disease severity, thousand grains weight, and relative yield reduction, we found that the more aggressive isolates were richer in lipid content. Therefore, we could define several spectroscopic markers (> C — H stretching modes in the 3000–2800 window, > C=O and — C — O vibrational modes of esters at 1765–1707 cm<sup>−1</sup> and 1474–900 cm<sup>−1</sup>, respectively),, mostly assigned to lipid content that could be associated with <i>F. graminearum</i> aggressiveness. All together, by the application of FTIR techniques and simple multivariate analyses, it was possible to gain significant insights into the phenotypic characterization of <i>F. graminearum</i> local isolates, and to establish the existence of a direct relationship between lipid content and fungal aggressiveness. Considering that lipids have a major role as mediators in the interaction between plants and fungi our results could represent an attractive outcome in the study of Fusarium pathogenesis. |
| format |
Articulo Articulo |
| author |
Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, María Alejandra Alconada Magliano, Teresa María |
| author_facet |
Fígoli, Cecilia Beatriz Rojo, Rodrigo Gasoni, Laura Kikot, Gisele Eleonora Leguizamón, Mariana Gamba, Raúl Ricardo Bosch, María Alejandra Alconada Magliano, Teresa María |
| author_sort |
Fígoli, Cecilia Beatriz |
| title |
Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| title_short |
Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| title_full |
Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| title_fullStr |
Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| title_full_unstemmed |
Characterization of <i>Fusarium graminearum</i> isolates recovered from wheat samples from Argentina by Fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| title_sort |
characterization of <i>fusarium graminearum</i> isolates recovered from wheat samples from argentina by fourier transform infrared spectroscopy: phenotypic diversity and detection of specific markers of aggressiveness |
| publishDate |
2017 |
| url |
http://sedici.unlp.edu.ar/handle/10915/104752 |
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