Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells

The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficult-to-transfect cells. It is well known that human, mouse, and rat skelet...

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Detalles Bibliográficos
Autores principales:	Pereyra, Andrea Soledad, Mykhaylyk, Olga, Falomir Lockhart, Eugenia, Taylor, Jackson Richard, Delbono, Osvaldo, Goya, Rodolfo Gustavo, Plank, Christian, Hereñú, Claudia Beatriz
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2016
Materias:	Bioquímica Ciencias Médicas Gene delivery Skeletal muscle Magnetic nanoparticles Adenoviral vectors Magnetofection Magneto-adenovectors
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/104734 http://hdl.handle.net/11336/51671
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-104734
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Bioquímica Ciencias Médicas Gene delivery Skeletal muscle Magnetic nanoparticles Adenoviral vectors Magnetofection Magneto-adenovectors
spellingShingle	Bioquímica Ciencias Médicas Gene delivery Skeletal muscle Magnetic nanoparticles Adenoviral vectors Magnetofection Magneto-adenovectors Pereyra, Andrea Soledad Mykhaylyk, Olga Falomir Lockhart, Eugenia Taylor, Jackson Richard Delbono, Osvaldo Goya, Rodolfo Gustavo Plank, Christian Hereñú, Claudia Beatriz Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
topic_facet	Bioquímica Ciencias Médicas Gene delivery Skeletal muscle Magnetic nanoparticles Adenoviral vectors Magnetofection Magneto-adenovectors
description	The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficult-to-transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction resistance in skeletal muscle cells. Expression of Green Fluorescent Protein and Insulin-like Growth Factor 1 was significantly enhanced after magnetofection with RAd-MNPs complexes in C2C12 myotubes in vitro and mouse skeletal muscle in vivo when compared to transduction with naked virus. These results provide evidence that magnetofection, mainly due to its membrane-receptor independent mechanism, constitutes a simple and effective alternative to current methods for gene transfer into traditionally hard-to-transfect biological models.
format	Articulo Articulo
author	Pereyra, Andrea Soledad Mykhaylyk, Olga Falomir Lockhart, Eugenia Taylor, Jackson Richard Delbono, Osvaldo Goya, Rodolfo Gustavo Plank, Christian Hereñú, Claudia Beatriz
author_facet	Pereyra, Andrea Soledad Mykhaylyk, Olga Falomir Lockhart, Eugenia Taylor, Jackson Richard Delbono, Osvaldo Goya, Rodolfo Gustavo Plank, Christian Hereñú, Claudia Beatriz
author_sort	Pereyra, Andrea Soledad
title	Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
title_short	Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
title_full	Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
title_fullStr	Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
title_full_unstemmed	Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
title_sort	magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/104734 http://hdl.handle.net/11336/51671
work_keys_str_mv	AT pereyraandreasoledad magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT mykhaylykolga magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT falomirlockharteugenia magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT taylorjacksonrichard magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT delbonoosvaldo magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT goyarodolfogustavo magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT plankchristian magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells AT herenuclaudiabeatriz magnetofectionenhancesadenoviralvectorbasedgenedeliveryinskeletalmusclecells
bdutipo_str	Repositorios
_version_	1764820442280886272

Magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells

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