Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance

We studied the effect of insulin resistance (IR) induced by administration of a fructose-rich diet (FRD) to normal Wistar rats for 21 days, upon islet plasma membrane calcium ATPases (PMCAs) and insulin secretion. FRD rats showed significantly higher triglyceride and insulin levels, insulin:glucose...

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Detalles Bibliográficos
Autores principales: Alzugaray, María Eugenia, García, María Elisa, Del Zotto, Héctor Herminio, Raschia, María Agustina, Palomeque, Julieta, Rossi, Juan Pablo Francisco, Gagliardino, Juan José, Flores, Luis Emilio
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2009
Materias:
Bioquímica
Ciencias Médicas
Calcium pumps
Insulin resistance
Insulin secretion
Pancreatic islets
PMCA isoforms
PMCAs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104727
http://hdl.handle.net/11336/94431
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104727
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Médicas
Calcium pumps
Insulin resistance
Insulin secretion
Pancreatic islets
PMCA isoforms
PMCAs
spellingShingle Bioquímica
Ciencias Médicas
Calcium pumps
Insulin resistance
Insulin secretion
Pancreatic islets
PMCA isoforms
PMCAs
Alzugaray, María Eugenia
García, María Elisa
Del Zotto, Héctor Herminio
Raschia, María Agustina
Palomeque, Julieta
Rossi, Juan Pablo Francisco
Gagliardino, Juan José
Flores, Luis Emilio
Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance
topic_facet Bioquímica
Ciencias Médicas
Calcium pumps
Insulin resistance
Insulin secretion
Pancreatic islets
PMCA isoforms
PMCAs
description We studied the effect of insulin resistance (IR) induced by administration of a fructose-rich diet (FRD) to normal Wistar rats for 21 days, upon islet plasma membrane calcium ATPases (PMCAs) and insulin secretion. FRD rats showed significantly higher triglyceride and insulin levels, insulin:glucose ratio and HOMA-IR index than controls. FRD islets released significantly more insulin in response to glucose and showed (a) marked changes in PMCA isoform protein content (decreased PMCA 2 and increased PMCA 3), (b) a decrease in total PMCAs activity, and (c) higher levels of cytosolic calcium [Ca2+]i. The lower PMCAs activity with the resultant increase in [Ca2+]i would favor the compensatory greater release of insulin necessary to cope with the IR state present in FRD rats and to maintain normal glucose homeostasis. Thus, changes in PMCAs activity and isoform expression play a modulatory role upon insulin secretion during long-term adaptation to an increased hormone demand.
format Articulo
Preprint
author Alzugaray, María Eugenia
García, María Elisa
Del Zotto, Héctor Herminio
Raschia, María Agustina
Palomeque, Julieta
Rossi, Juan Pablo Francisco
Gagliardino, Juan José
Flores, Luis Emilio
author_facet Alzugaray, María Eugenia
García, María Elisa
Del Zotto, Héctor Herminio
Raschia, María Agustina
Palomeque, Julieta
Rossi, Juan Pablo Francisco
Gagliardino, Juan José
Flores, Luis Emilio
author_sort Alzugaray, María Eugenia
title Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance
title_short Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance
title_full Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance
title_fullStr Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance
title_full_unstemmed Changes in islet plasma membrane calcium-ATPase activity and isoform expression induced by insulin resistance
title_sort changes in islet plasma membrane calcium-atpase activity and isoform expression induced by insulin resistance
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/104727
http://hdl.handle.net/11336/94431
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