Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation

Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simul...

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Autores principales: Rengifo Herrera, Julián Andrés, Castaño, Olga L., Sanabria, Irma Janeth
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104624
http://hdl.handle.net/11336/4932
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104624
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
spellingShingle Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
Rengifo Herrera, Julián Andrés
Castaño, Olga L.
Sanabria, Irma Janeth
Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
topic_facet Química
Ecología
Photo-fenton disinfection
Salmonella typhimurium inactivation
VBNC state
Viability and cultivability
description Culturability and viability techniques such as plate count on solid agar (PC), Most Probable Number (MPN) and Direct Viable Count-Fluorescence in Situ Hybridation (DVC FISH) were used to study the inactivation of Salmonella typhimurium by photo-Fenton process at pH 5.5. In the presence of only simulated solar irradiation (500 W·m−2), S. typhimurim showed that both culturability measured by MPN and viability (measured by DVC FISH) underwent just a slight decreasing of 2 and 1 log respectively after 240 min of light exposition while culturability measured by PC did not show any change. Results after 48 h of dark conditions did not reveal re-growth. However, when experiment was carried out in the presence of 2 mg L−1 of Fe3+ and 20 mg L−1 of H2O2 and pH 5.5, culturability was strongly affected after 240 min of simulated solar irradiation; nevertheless, viability was only slightly altered (~1 log). During dark period of 48 h changes on culturability and viability were not observed. On the other hand, it was also found that sugar metabolism was affected rather the amino-acids in S. typhimurium cells irradiated at different times upon photo-Fenton conditions. These findings might suggest for the first time that photo-Fenton process at pH 5.5 could induce viable but nonculturable state (VBNC) on waterborne S. typhimurium and that probably sugar metabolism damage could activate the VBNC state.
format Articulo
Articulo
author Rengifo Herrera, Julián Andrés
Castaño, Olga L.
Sanabria, Irma Janeth
author_facet Rengifo Herrera, Julián Andrés
Castaño, Olga L.
Sanabria, Irma Janeth
author_sort Rengifo Herrera, Julián Andrés
title Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_short Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_full Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_fullStr Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_full_unstemmed Culturability and viability of Salmonella typhimurium during photo-Fenton process at pH 5.5 under solar simulated irradiation
title_sort culturability and viability of salmonella typhimurium during photo-fenton process at ph 5.5 under solar simulated irradiation
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/104624
http://hdl.handle.net/11336/4932
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AT castanoolgal culturabilityandviabilityofsalmonellatyphimuriumduringphotofentonprocessatph55undersolarsimulatedirradiation
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bdutipo_str Repositorios
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