Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen

<i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> ex...

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Detalles Bibliográficos
Autores principales: Bottero, Daniela, Zurita, María Eugenia, Gaillard, María Emilia, Bartel, Erika Belén, Vercellini, María Clara, Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104593
http://hdl.handle.net/11336/96975
https://aem.asm.org/content/84/4/e01877-17
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104593
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
spellingShingle Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
Bottero, Daniela
Zurita, María Eugenia
Gaillard, María Emilia
Bartel, Erika Belén
Vercellini, María Clara
Hozbor, Daniela Flavia
Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
topic_facet Ciencias Exactas
Biología
Bordetella bronchiseptica
outer membrane vesicles
phenotypic phases
vaccine
description <i>Bordetella bronchiseptica</i>, a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i> experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase <i>B. bronchiseptica</i> (OMVBbvir⁺) protected mice against sublethal infections with different <i>B. bronchiseptica</i> strains, two isolated from farm animals and one isolated from a human patient. In all infections, we observed that the <i>B. bronchiseptica</i> loads were significantly reduced in the lungs of vaccinated animals; the lung-recovered CFU were decreased by ≥4 log units, compared with those detected in the lungs of nonimmunized animals (P < 0.001). In the OMVBbvir⁺-immunized mice, we detected IgG antibody titers against <i>B. bronchiseptica</i> whole-cell lysates, along with an immune serum having bacterial killing activity that both recognized <i>B. bronchiseptica</i> lipopolysaccharides and polypeptides such as GroEL and outer membrane protein C (OMPc) and demonstrated an essential protective capacity against <i>B. bronchiseptica</i> infection, as detected by passive in vivo transfer experiments. Stimulation of cultured splenocytes from immunized mice with OMVBbvir⁺ resulted in interleukin 5 (IL-5), gamma interferon (IFN-γ), and IL-17 production, indicating that the vesicles induced mixed Th2, Th1, and Th17 T-cell immune responses. We detected, by adoptive transfer assays, that spleen cells from OMVBbvir⁺-immunized mice also contributed to the observed protection against <i>B. bronchiseptica</i> infection. OMVs from avirulent-phase <i>B. bronchiseptica</i> and the resulting induced immune sera were also able to protect mice against <i>B. bronchiseptica</i> infection.
format Articulo
Articulo
author Bottero, Daniela
Zurita, María Eugenia
Gaillard, María Emilia
Bartel, Erika Belén
Vercellini, María Clara
Hozbor, Daniela Flavia
author_facet Bottero, Daniela
Zurita, María Eugenia
Gaillard, María Emilia
Bartel, Erika Belén
Vercellini, María Clara
Hozbor, Daniela Flavia
author_sort Bottero, Daniela
title Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_short Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_full Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_fullStr Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_full_unstemmed Membrane vesicles derived from <i>Bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
title_sort membrane vesicles derived from <i>bordetella bronchiseptica</i>: active constituent of a new vaccine against infections caused by this pathogen
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/104593
http://hdl.handle.net/11336/96975
https://aem.asm.org/content/84/4/e01877-17
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