Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains

A new isolate of the <i>Spodoptera frugiperda granulovirus</i>, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found in betabaculoviruses, 19 of which are present only in GVs clo...

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Detalles Bibliográficos
Autores principales: Ferrelli, María Leticia, Pidre, Matías Luis, Ghiringhelli, Pablo Daniel, Torres, Sofía, Fabre, María Laura, Masson, Tomás, Cedola, Maia Tatiana, Sciocco, Alicia Inés, Romanowski, Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104532
http://hdl.handle.net/11336/96973
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202598
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104532
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
spellingShingle Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
Ferrelli, María Leticia
Pidre, Matías Luis
Ghiringhelli, Pablo Daniel
Torres, Sofía
Fabre, María Laura
Masson, Tomás
Cedola, Maia Tatiana
Sciocco, Alicia Inés
Romanowski, Víctor
Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
topic_facet Ciencias Exactas
Biología
Baculovirus
Granulovirus
Spodoptera frugiperda
genome
description A new isolate of the <i>Spodoptera frugiperda granulovirus</i>, SfGV ARG, was completely sequenced and analyzed. The SfGV ARG genome is 139,812 bp long and encodes 151 putative open reading frames. Of these ORFs, 56 were found in betabaculoviruses, 19 of which are present only in GVs closely related to SfGV. Seven ORFs found homologs in this small GV group and also in noctuid NPVs. ORF066 codes a 74 amino acid protein, overlapped with <i>nudix</i> gene, with several homologs in baculovirus, found by tblastn search. Comparison with the genome of the Colombian isolate SfGV VG008 resulted in SfGV being 1101 bp smaller and lacking a homologue of VG008 ORF084, which codes for Lef-7. However, we found that ORF051 shows remote homology to Lef-7 proteins. Moreover, analysis of ORF051 along with Lef-7 proteins coded by a group of noctuid specific GVs and NPVs indicated that Lef-7 proteins coded by these viruses include three F-box domains in contrast to the single one reported for AcMNPV Lef-7. SfGV ARG genome also contains a split photolyase as a distinct feature not found in VG008. BlastX analysis revealed that a complete photolyase is coded considering a putative frameshift in a poly-A tract, which resembles known slippery sequences involved in programmed ribosome frameshifting.
format Articulo
Articulo
author Ferrelli, María Leticia
Pidre, Matías Luis
Ghiringhelli, Pablo Daniel
Torres, Sofía
Fabre, María Laura
Masson, Tomás
Cedola, Maia Tatiana
Sciocco, Alicia Inés
Romanowski, Víctor
author_facet Ferrelli, María Leticia
Pidre, Matías Luis
Ghiringhelli, Pablo Daniel
Torres, Sofía
Fabre, María Laura
Masson, Tomás
Cedola, Maia Tatiana
Sciocco, Alicia Inés
Romanowski, Víctor
author_sort Ferrelli, María Leticia
title Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_short Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_full Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_fullStr Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_full_unstemmed Genomic analysis of an Argentinean isolate of <i>Spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for Lef-7 proteins with three F-box domains
title_sort genomic analysis of an argentinean isolate of <i>spodoptera frugiperda granulovirus</i> reveals that various baculoviruses code for lef-7 proteins with three f-box domains
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/104532
http://hdl.handle.net/11336/96973
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0202598
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