Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats

Bacteria belonging to the genus <i>Acinetobacter</i> have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among <i>Acinetob...

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Detalles Bibliográficos
Autores principales: Salto, Ileana Paula, Torres Tejerizo, Gonzalo Arturo, Wibberg, Daniel, Pühler, Alfred, Schlüter, Andreas, Pistorio, Mariano
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Exactas
Biología
Acinetobacter
plasmids
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104526
http://hdl.handle.net/11336/96690
https://www.nature.com/articles/s41598-018-26180-3
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104526
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Acinetobacter
plasmids
spellingShingle Ciencias Exactas
Biología
Acinetobacter
plasmids
Salto, Ileana Paula
Torres Tejerizo, Gonzalo Arturo
Wibberg, Daniel
Pühler, Alfred
Schlüter, Andreas
Pistorio, Mariano
Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
topic_facet Ciencias Exactas
Biología
Acinetobacter
plasmids
description Bacteria belonging to the genus <i>Acinetobacter</i> have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among <i>Acinetobacter</i> strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental <i>Acinetobacter</i> spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all <i>Acinetobacter</i> Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQ<sub>Aci</sub>, composed exclusively of <i>Acinetobacter</i> relaxases. Functional analysis of proteins belonging to this group showed that they behaved differently when mobilized using helper plasmids belonging to different incompatibility groups.
format Articulo
Articulo
author Salto, Ileana Paula
Torres Tejerizo, Gonzalo Arturo
Wibberg, Daniel
Pühler, Alfred
Schlüter, Andreas
Pistorio, Mariano
author_facet Salto, Ileana Paula
Torres Tejerizo, Gonzalo Arturo
Wibberg, Daniel
Pühler, Alfred
Schlüter, Andreas
Pistorio, Mariano
author_sort Salto, Ileana Paula
title Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_short Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_full Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_fullStr Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_full_unstemmed Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_sort comparative genomic analysis of <i>acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/104526
http://hdl.handle.net/11336/96690
https://www.nature.com/articles/s41598-018-26180-3
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