Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages

Shiga toxigenic Escherichia coli O157 is the leading cause of hemolytic uremic syndrome (HUS) worldwide. The frequencies of stx genotypes and the incidences of O157-related illness and HUS vary significantly between Argentina and Australia. Locusspecific polymorphism analysis revealed that lineage I...

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Autores principales: Mellor, Glen E., Sim, Eby M., Barlow, Robert S., D'Astek, Beatriz A., Galli, Lucía, Chinen, Isabel, Rivas, Marta, Gobius, Kari S.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Veterinarias
Biología
Escherichia coli O157
LSPA-6
Phylogenetic
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104496
http://hdl.handle.net/11336/79014
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104496
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Biología
Escherichia coli O157
LSPA-6
Phylogenetic
spellingShingle Ciencias Veterinarias
Biología
Escherichia coli O157
LSPA-6
Phylogenetic
Mellor, Glen E.
Sim, Eby M.
Barlow, Robert S.
D'Astek, Beatriz A.
Galli, Lucía
Chinen, Isabel
Rivas, Marta
Gobius, Kari S.
Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
topic_facet Ciencias Veterinarias
Biología
Escherichia coli O157
LSPA-6
Phylogenetic
description Shiga toxigenic Escherichia coli O157 is the leading cause of hemolytic uremic syndrome (HUS) worldwide. The frequencies of stx genotypes and the incidences of O157-related illness and HUS vary significantly between Argentina and Australia. Locusspecific polymorphism analysis revealed that lineage I/II (LI/II) E. coli O157 isolates were most prevalent in Argentina (90%) and Australia (88%). Argentinean LI/II isolates were shown to belong to clades 4 (28%) and 8 (72%), while Australian LI/II isolates were identified as clades 6 (15%), 7 (83%), and 8 (2%). Clade 8 was significantly associated with Shiga toxin bacteriophage insertion (SBI) type stx2 (locus of insertion, argW) in Argentinean isolates (P<0.0001). In Argentinean LI/II strains, stx2 is carried by a prophage inserted at argW, whereas in Australian LI/II strains the argW locus is occupied by the novel stx1 prophage. In both Argentinean and Australian LI/II strains, stx2c is almost exclusively carried by a prophage inserted at sbcB. However, alternative q933- or q21-related alleles were identified in the Australian stx2c prophage. Argentinean LI/II isolates were also distinguished from Australian isolates by the presence of the putative virulence determinant ECSP_3286 and the predominance of motile O157:H7 strains. Characteristics common to both Argentinean and Australian LI/II O157 strains included the presence of putative virulence determinants (ECSP_3620, ECSP_0242, ECSP_2687, ECSP_2870, and ECSP_2872) and the predominance of the tir255T allele. These data support further understanding of O157 phylogeny and may foster greater insight into the differential virulence of O157 lineages. © 2012, American Society for Microbiology.
format Articulo
Articulo
author Mellor, Glen E.
Sim, Eby M.
Barlow, Robert S.
D'Astek, Beatriz A.
Galli, Lucía
Chinen, Isabel
Rivas, Marta
Gobius, Kari S.
author_facet Mellor, Glen E.
Sim, Eby M.
Barlow, Robert S.
D'Astek, Beatriz A.
Galli, Lucía
Chinen, Isabel
Rivas, Marta
Gobius, Kari S.
author_sort Mellor, Glen E.
title Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
title_short Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
title_full Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
title_fullStr Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
title_full_unstemmed Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
title_sort phylogenetically related argentinean and australian escherichia coli o157 isolates are distinguished by virulence clades and alternative shiga toxin 1 and 2 prophages
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/104496
http://hdl.handle.net/11336/79014
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