A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology

The eggs of the freshwater <i>Pomacea</i> apple snails develop above the water level, exposed to varied physical and biological stressors. Their high hatching success seems to be linked to their proteins or perivitellins, which surround the developing embryo providing nutrients, sunscree...

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Autores principales: Ituarte, Santiago, Brola, Tabata Romina, Fernández, Patricia Elena, Mu, Huawei, Qiu, Jian Wen, Heras, Horacio, Dreon, Marcos Sebastián
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Veterinarias
Ciencias Médicas
Biología
lectins
eggs
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104458
http://hdl.handle.net/11336/97864
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198361
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104458
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Ciencias Médicas
Biología
lectins
eggs
spellingShingle Ciencias Veterinarias
Ciencias Médicas
Biología
lectins
eggs
Ituarte, Santiago
Brola, Tabata Romina
Fernández, Patricia Elena
Mu, Huawei
Qiu, Jian Wen
Heras, Horacio
Dreon, Marcos Sebastián
A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
topic_facet Ciencias Veterinarias
Ciencias Médicas
Biología
lectins
eggs
description The eggs of the freshwater <i>Pomacea</i> apple snails develop above the water level, exposed to varied physical and biological stressors. Their high hatching success seems to be linked to their proteins or perivitellins, which surround the developing embryo providing nutrients, sunscreens and varied defenses. The defensive mechanism has been unveiled in <i>P. canaliculata</i> and <i>P. maculata</i> eggs, where their major perivitellins are pigmented, non-digestible and provide a warning coloration while another perivitellin acts as a toxin. In <i>P. scalaris</i>, a species sympatric to the former, the defense strategy seems different, since no toxin was found and the major perivitellin, PsSC, while also colored and non-digestible, is a carbohydrate-binding protein. In this study we examine the structure and function of PsSC by sequencing its subunits, characterizing its carbohydrate binding profile and evaluating its effect on gut cells. Whereas cDNA sequencing and database search showed no lectin domain, glycan array carbohydrate binding profile revealed a strong specificity for glycosphingolipids and ABO group antigens. Moreover, PsSC agglutinated bacteria in a dose-dependent manner. Inspired on the defensive properties of seed lectins we evaluated the effects of PsSC on intestinal cells both <i>in vitro</i> (Caco-2 and IEC-6 cells) and in the gastrointestinal tract of rats. PsSC binds to Caco-2 cell membranes without reducing its viability, while a PsSC-containing diet temporarily induces large epithelium alterations and an increased absorptive surface. Based on these results, we propose that PsSC is involved in embryo defenses by altering the gut morphophysiology of potential predators, a convergent role to plant defensive lectins.
format Articulo
Articulo
author Ituarte, Santiago
Brola, Tabata Romina
Fernández, Patricia Elena
Mu, Huawei
Qiu, Jian Wen
Heras, Horacio
Dreon, Marcos Sebastián
author_facet Ituarte, Santiago
Brola, Tabata Romina
Fernández, Patricia Elena
Mu, Huawei
Qiu, Jian Wen
Heras, Horacio
Dreon, Marcos Sebastián
author_sort Ituarte, Santiago
title A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
title_short A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
title_full A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
title_fullStr A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
title_full_unstemmed A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
title_sort lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/104458
http://hdl.handle.net/11336/97864
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0198361
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