Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

Antibodies are important tools for experimental research and medical applications. Most antibodies are composed of two heavy and two light chains. Both chains contribute to the antigen-binding site which is usually flat or concave. In addition to these conventional antibodies, llamas, other camelids...

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Autores principales: Wesolowski, Janusz, Alzogaray, Vanina Andrea, Reyelt, Jan, Unger, Mandy, Juarez, Karla, Urrutia, Mariela, Cauerhff, Ana Albina, Danquah, Welbeck, Rissiek, Björn, Scheuplein, Felix, Schwarz, Nicole, Adriouch, Sahil, Boyer, Olivier, Seman, Michel, Licea, Alexei, Serreze, David V., Goldbaum, Fernando Alberto, Haag, Friedrich, Koch Nolte, Friedrich
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Biología
Enzyme inhibitors
Nanobody
Recombinant antibodies
Single domain antibodies
VHH
Virus neutralization
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104354
http://hdl.handle.net/11336/36849
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104354
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Enzyme inhibitors
Nanobody
Recombinant antibodies
Single domain antibodies
VHH
Virus neutralization
spellingShingle Biología
Enzyme inhibitors
Nanobody
Recombinant antibodies
Single domain antibodies
VHH
Virus neutralization
Wesolowski, Janusz
Alzogaray, Vanina Andrea
Reyelt, Jan
Unger, Mandy
Juarez, Karla
Urrutia, Mariela
Cauerhff, Ana Albina
Danquah, Welbeck
Rissiek, Björn
Scheuplein, Felix
Schwarz, Nicole
Adriouch, Sahil
Boyer, Olivier
Seman, Michel
Licea, Alexei
Serreze, David V.
Goldbaum, Fernando Alberto
Haag, Friedrich
Koch Nolte, Friedrich
Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
topic_facet Biología
Enzyme inhibitors
Nanobody
Recombinant antibodies
Single domain antibodies
VHH
Virus neutralization
description Antibodies are important tools for experimental research and medical applications. Most antibodies are composed of two heavy and two light chains. Both chains contribute to the antigen-binding site which is usually flat or concave. In addition to these conventional antibodies, llamas, other camelids, and sharks also produce antibodies composed only of heavy chains. The antigen-binding site of these unusual heavy chain antibodies (hcAbs) is formed only by a single domain, designated VHH in camelid hcAbs and VNAR in shark hcAbs. VHH and VNAR are easily produced as recombinant proteins, designated single domain antibodies (sdAbs) or nanobodies. The CDR3 region of these sdAbs possesses the extraordinary capacity to form long fingerlike extensions that can extend into cavities on antigens, e.g., the active site crevice of enzymes. Other advantageous features of nanobodies include their small size, high solubility, thermal stability, refolding capacity, and good tissue penetration in vivo. Here we review the results of several recent proof-of-principle studies that open the exciting perspective of using sdAbs for modulating immune functions and for targeting toxins and microbes.
format Articulo
Articulo
author Wesolowski, Janusz
Alzogaray, Vanina Andrea
Reyelt, Jan
Unger, Mandy
Juarez, Karla
Urrutia, Mariela
Cauerhff, Ana Albina
Danquah, Welbeck
Rissiek, Björn
Scheuplein, Felix
Schwarz, Nicole
Adriouch, Sahil
Boyer, Olivier
Seman, Michel
Licea, Alexei
Serreze, David V.
Goldbaum, Fernando Alberto
Haag, Friedrich
Koch Nolte, Friedrich
author_facet Wesolowski, Janusz
Alzogaray, Vanina Andrea
Reyelt, Jan
Unger, Mandy
Juarez, Karla
Urrutia, Mariela
Cauerhff, Ana Albina
Danquah, Welbeck
Rissiek, Björn
Scheuplein, Felix
Schwarz, Nicole
Adriouch, Sahil
Boyer, Olivier
Seman, Michel
Licea, Alexei
Serreze, David V.
Goldbaum, Fernando Alberto
Haag, Friedrich
Koch Nolte, Friedrich
author_sort Wesolowski, Janusz
title Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
title_short Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
title_full Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
title_fullStr Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
title_full_unstemmed Single domain antibodies: promising experimental and therapeutic tools in infection and immunity
title_sort single domain antibodies: promising experimental and therapeutic tools in infection and immunity
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/104354
http://hdl.handle.net/11336/36849
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