Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L

<b>Introduction</b>: Volcanism is an important natural origin of Arsenic (As) contamination. <b>Aims</b>: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Desi...

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Autores principales: Marsa, Silvana Mariel, Rossi, Franco Rubén, Valdez, Hugo Alberto, Gomez Casati, Diego Fabian, Zirulnik, Fanny
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Biología
Química
Arsenic
Soybean
Oxidative stress
Antioxidant defense
Genotoxicity
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104311
http://hdl.handle.net/11336/16091
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104311
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Química
Arsenic
Soybean
Oxidative stress
Antioxidant defense
Genotoxicity
spellingShingle Biología
Química
Arsenic
Soybean
Oxidative stress
Antioxidant defense
Genotoxicity
Marsa, Silvana Mariel
Rossi, Franco Rubén
Valdez, Hugo Alberto
Gomez Casati, Diego Fabian
Zirulnik, Fanny
Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L
topic_facet Biología
Química
Arsenic
Soybean
Oxidative stress
Antioxidant defense
Genotoxicity
description <b>Introduction</b>: Volcanism is an important natural origin of Arsenic (As) contamination. <b>Aims</b>: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Design: The plants were separated into two groups (Control and As-treated ones) until they were exposed to As (2.5 and 5 ppm) at day 10. <b>Methodology</b>: Biochemical, molecular and cytogenetic determinations were carried out. <b>Results</b>: Arsenic treated plants show growth abnormalities. The antioxidant defenses and oxidative parameters in roots and leaves showed different tissue-specific responses. Whereas leaf tissues display an active antioxidant defense, root cells are unable to produce defense molecules and show oxidative stress. Moreover, the analysis of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase, showed higher activity in leaves than in roots, except for isocitrate dehydrogenase. The expression of GR and MAPK decreased significantly in all the treatments during the time curve. ICDH showed an increase in its expression with 24 hours of treatment and falls at 72 hours compared to their controls. Genotoxicity assays showed a decrease of mitotic index (MI) and the presence of altered nucleus, in samples exposed for 72 hours. <b>Conclusion</b>: Our results suggest that the physiological, biochemical, molecular and cytogenetic behavior of the plants depends on the As concentration and the length of the exposure.
format Articulo
Articulo
author Marsa, Silvana Mariel
Rossi, Franco Rubén
Valdez, Hugo Alberto
Gomez Casati, Diego Fabian
Zirulnik, Fanny
author_facet Marsa, Silvana Mariel
Rossi, Franco Rubén
Valdez, Hugo Alberto
Gomez Casati, Diego Fabian
Zirulnik, Fanny
author_sort Marsa, Silvana Mariel
title Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L
title_short Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L
title_full Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L
title_fullStr Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L
title_full_unstemmed Study of oxidative stress and genotoxicity by arsenic contamination in Glycine <i>max.</i> L
title_sort study of oxidative stress and genotoxicity by arsenic contamination in glycine <i>max.</i> l
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/104311
http://hdl.handle.net/11336/16091
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AT gomezcasatidiegofabian studyofoxidativestressandgenotoxicitybyarseniccontaminationinglycineimaxil
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