The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection

The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against Pseudomonas syringae pv. tomato (Pst). The tomato genome has two Pti1 genes, referred to as Pti1a and Pti1b. A hairpin-Pti1 (hpPti1) construct was developed and was used to genera...

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Autores principales: Schwizer, Simon, Kraus, Christine M., Dunham, Diane M., Zheng, Yi, Fernández Pozo, Noé, Pombo, Marina Alejandra, Fei, Zhangjun, Chakravarthy, Suma, Martin, Gregory B.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Pattern-triggered immunity
Pti1
Ros production
Tomato defense
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104188
http://hdl.handle.net/11336/65416
https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-17-0056-R
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104188
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Pattern-triggered immunity
Pti1
Ros production
Tomato defense
spellingShingle Biología
Pattern-triggered immunity
Pti1
Ros production
Tomato defense
Schwizer, Simon
Kraus, Christine M.
Dunham, Diane M.
Zheng, Yi
Fernández Pozo, Noé
Pombo, Marina Alejandra
Fei, Zhangjun
Chakravarthy, Suma
Martin, Gregory B.
The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
topic_facet Biología
Pattern-triggered immunity
Pti1
Ros production
Tomato defense
description The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against Pseudomonas syringae pv. tomato (Pst). The tomato genome has two Pti1 genes, referred to as Pti1a and Pti1b. A hairpin-Pti1 (hpPti1) construct was developed and was used to generate two independent stable transgenic tomato lines that had reduced transcript abundance of both genes. In response to P. syringae pv. tomato inoculation, these hpPti1 plants developed more severe disease symptoms, supported higher bacterial populations, and had reduced transcript accumulation of PTI-associated genes, as compared with wild-type plants. In response to two flagellin-derived peptides, the hpPti1 plants produced lesser amounts of reactive oxygen species (ROS) but showed no difference in mitogen-activated protein kinase (MAPK). Synthetic Pti1a and Pti1b genes designed to avoid silencing were transiently expressed in the hpPti1 plants and restored the ability of the plants to produce wild-type levels of ROS. Our results identify a new component of PTI in tomato that, because it affects ROS production but not MAPK signaling, appears to act early in the immune response.
format Articulo
Articulo
author Schwizer, Simon
Kraus, Christine M.
Dunham, Diane M.
Zheng, Yi
Fernández Pozo, Noé
Pombo, Marina Alejandra
Fei, Zhangjun
Chakravarthy, Suma
Martin, Gregory B.
author_facet Schwizer, Simon
Kraus, Christine M.
Dunham, Diane M.
Zheng, Yi
Fernández Pozo, Noé
Pombo, Marina Alejandra
Fei, Zhangjun
Chakravarthy, Suma
Martin, Gregory B.
author_sort Schwizer, Simon
title The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_short The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_full The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_fullStr The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_full_unstemmed The tomato kinase Pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae Infection
title_sort tomato kinase pti1 contributes to production of reactive oxygen species in response to two flagellin-derived peptides and promotes resistance to pseudomonas syringae infection
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/104188
http://hdl.handle.net/11336/65416
https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-17-0056-R
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