Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment

Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and...

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Autores principales: Felice, Juan Ignacio, Schurman, León, McCarthy, Antonio Desmond, Sedlinsky, Claudia, Aguirre, José Ignacio, Cortizo, Ana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/104013
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-104013
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
spellingShingle Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
topic_facet Biología
Metabolic syndrome
Fructose
Bone architecture
Osteoblasts
Metformin
description Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS.
format Articulo
Articulo
author Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author_facet Felice, Juan Ignacio
Schurman, León
McCarthy, Antonio Desmond
Sedlinsky, Claudia
Aguirre, José Ignacio
Cortizo, Ana María
author_sort Felice, Juan Ignacio
title Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_short Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_full Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_fullStr Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_full_unstemmed Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
title_sort effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/104013
work_keys_str_mv AT felicejuanignacio effectsoffructoseinducedmetabolicsyndromeonratskeletalcellsandtissueandtheirresponsestometformintreatment
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bdutipo_str Repositorios
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