Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, and 1 mM EPA. The effect of EPA was evaluated on cumulus‐oocyte complexes (COC)...

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Autores principales: Nikoloff, Noelia, Pascua, Ana Malen, Anchordoquy, Juan Mateo, Anchordoquy, Juan Patricio, Sirini, Matías Ángel, Seoane, Analía Isabel, Furnus, Cecilia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
EPA
MTT
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103935
https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.10746
Aporte de:
id I19-R120-10915-103935
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Single cell gel electrophoresis
Apoptosis
Bovine
EPA
MTT
spellingShingle Ciencias Veterinarias
Single cell gel electrophoresis
Apoptosis
Bovine
EPA
MTT
Nikoloff, Noelia
Pascua, Ana Malen
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Sirini, Matías Ángel
Seoane, Analía Isabel
Furnus, Cecilia Cristina
Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
topic_facet Ciencias Veterinarias
Single cell gel electrophoresis
Apoptosis
Bovine
EPA
MTT
description The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, and 1 mM EPA. The effect of EPA was evaluated on cumulus‐oocyte complexes (COC) by oocyte maturation (cumulus expansion area and oocyte nuclear maturation), genotoxicity [single cell gel electrophoresis (SCGE)], and cytotoxicity (apoptosis, viability, and MTT assays) end points. The maturation parameters were affected by exposure of COC to different EPA concentrations in the IVM medium. Cumulus expansion area increased in the presence of 1 nM EPA (P < 0.05) whereas addition of 1 nM EPA (P < 0.05) decreased cumulus expansion after 24 h of IVM. Moreover, the maturation rate significantly decreased when 1 mM of EPA was assayed (P < 0.001). EPA at 1 nM induced genotoxic and cytotoxic effects on bovine cumulus cells (CC) and primary DNA lesions (P < 0.001). A significant increase in the frequency of apoptotic (P < 0.01) and necrotic (P < 0.001) cells was observed after 24 h of treatment with 1 nM, 1 μM, and 1 mM EPA. Mitochondrial activity was altered with 1 mM EPA (P < 0.001). We inferred that optimal oocyte quality was partially dependent on the presence of adequate EPA concentrations; EPA could be beneficial to improve oocyte quality in the maturation process, because low concentration tested (1 nM EPA) improved cumulus expansion.
format Articulo
Articulo
author Nikoloff, Noelia
Pascua, Ana Malen
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Sirini, Matías Ángel
Seoane, Analía Isabel
Furnus, Cecilia Cristina
author_facet Nikoloff, Noelia
Pascua, Ana Malen
Anchordoquy, Juan Mateo
Anchordoquy, Juan Patricio
Sirini, Matías Ángel
Seoane, Analía Isabel
Furnus, Cecilia Cristina
author_sort Nikoloff, Noelia
title Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
title_short Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
title_full Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
title_fullStr Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
title_full_unstemmed Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
title_sort effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103935
https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.10746
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