Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation
In order to study the mechanisms regulating the phenanthrene degradation pathway and the intermediate-metabolite accumulation in strain <i>S. paucimobilis</i> 20006FA, we sequenced the genome and compared the genome-based predictions to experimental proteomic analyses. Physiological stud...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2017
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/103387 http://link.springer.com/article/10.1007/s11274-017-2391-6/fulltext.html |
| Aporte de: |
| id |
I19-R120-10915-103387 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Strain 20006FA Genomics HNA accumulation Phenanthrene pathway Proteomics |
| spellingShingle |
Ciencias Exactas Strain 20006FA Genomics HNA accumulation Phenanthrene pathway Proteomics Rumi Macchi Zubiaurre, Alejandra Martinez, M. Neme Tauil, R. M. Valacco, M. P. Morelli, Irma Susana Coppotelli, Bibiana Marina Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| topic_facet |
Ciencias Exactas Strain 20006FA Genomics HNA accumulation Phenanthrene pathway Proteomics |
| description |
In order to study the mechanisms regulating the phenanthrene degradation pathway and the intermediate-metabolite accumulation in strain <i>S. paucimobilis</i> 20006FA, we sequenced the genome and compared the genome-based predictions to experimental proteomic analyses. Physiological studies indicated that the degradation involved the salicylate and protocatechuate pathways, reaching 56.3% after 15 days. Furthermore, the strain degraded other polycyclic aromatic hydrocarbons (PAH) such as anthracene (13.1%), dibenzothiophene (76.3%), and fluoranthene. The intermediate metabolite 1-hydroxy2-naphthoic acid (HNA) accumulated during phenanthrene catabolism and inhibited both bacterial growth and phenanthrene degradation, but exogenous-HNA addition did not affect further degradation. Genomic analysis predicted 126 putative genes encoding enzymes for all the steps of phenanthrene degradation, which loci could also participate in the metabolism of other PAH. Proteomic analysis identified enzymes involved in 19 of the 23 steps needed for the transformation of phenanthrene to trichloroacetic-acid intermediates that were upregulated in phenanthrene cultures relative to the levels in glucose cultures.
Moreover, the protein-induction pattern was temporal, varying between 24 and 96 h during phenanthrene degradation, with most catabolic proteins being overexpressed at 96 h—e. g., the biphenyl dioxygenase and a multispecies (2Fe–2S)-binding protein. These results provided the first clues about regulation of expression of phenanthrene degradative enzymes in strain 20006FA and enabled an elucidation of the metabolic pathway utilized by the bacterium. To our knowledge the present work represents the first investigation of genomic, proteomic, and physiological studies of a PAH-degrading <i>Sphingomonas</i> strain. |
| format |
Articulo Articulo |
| author |
Rumi Macchi Zubiaurre, Alejandra Martinez, M. Neme Tauil, R. M. Valacco, M. P. Morelli, Irma Susana Coppotelli, Bibiana Marina |
| author_facet |
Rumi Macchi Zubiaurre, Alejandra Martinez, M. Neme Tauil, R. M. Valacco, M. P. Morelli, Irma Susana Coppotelli, Bibiana Marina |
| author_sort |
Rumi Macchi Zubiaurre, Alejandra |
| title |
Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| title_short |
Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| title_full |
Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| title_fullStr |
Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| title_full_unstemmed |
Insights into the genome and proteome of <i>Sphingomonas paucimobilis</i> strain 20006FA involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| title_sort |
insights into the genome and proteome of <i>sphingomonas paucimobilis</i> strain 20006fa involved in the regulation of polycyclic aromatic hydrocarbon degradation |
| publishDate |
2017 |
| url |
http://sedici.unlp.edu.ar/handle/10915/103387 http://link.springer.com/article/10.1007/s11274-017-2391-6/fulltext.html |
| work_keys_str_mv |
AT rumimacchizubiaurrealejandra insightsintothegenomeandproteomeofisphingomonaspaucimobilisistrain20006fainvolvedintheregulationofpolycyclicaromatichydrocarbondegradation AT martinezm insightsintothegenomeandproteomeofisphingomonaspaucimobilisistrain20006fainvolvedintheregulationofpolycyclicaromatichydrocarbondegradation AT nemetauilrm insightsintothegenomeandproteomeofisphingomonaspaucimobilisistrain20006fainvolvedintheregulationofpolycyclicaromatichydrocarbondegradation AT valaccomp insightsintothegenomeandproteomeofisphingomonaspaucimobilisistrain20006fainvolvedintheregulationofpolycyclicaromatichydrocarbondegradation AT morelliirmasusana insightsintothegenomeandproteomeofisphingomonaspaucimobilisistrain20006fainvolvedintheregulationofpolycyclicaromatichydrocarbondegradation AT coppotellibibianamarina insightsintothegenomeandproteomeofisphingomonaspaucimobilisistrain20006fainvolvedintheregulationofpolycyclicaromatichydrocarbondegradation |
| bdutipo_str |
Repositorios |
| _version_ |
1764820441905496065 |