Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species

Thermal inactivation of peroxidase (POD) and lipoxygenase (LOX), both enzymes present in broccoli and Brussels sprouts, is required before freezing, to obtain high-quality precooked frozen vegetables. Rate constants of a 1st-order biphasic model for the heat-labile and heat-resistant POD and LOX iso...

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Detalles Bibliográficos
Autores principales: Pérez Calderón, John Freddy, Califano, Alicia Noemí, Santos, María Victoria, Zaritzky, Noemí Elisabet
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Thermal inactivation kinetics
Lipoxygenase
Peroxidase
Precooked frozen Brassicas
Quality attributes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103096
https://onlinelibrary.wiley.com/doi/10.1111/1750-3841.13717
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103096
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Thermal inactivation kinetics
Lipoxygenase
Peroxidase
Precooked frozen Brassicas
Quality attributes
spellingShingle Ciencias Exactas
Thermal inactivation kinetics
Lipoxygenase
Peroxidase
Precooked frozen Brassicas
Quality attributes
Pérez Calderón, John Freddy
Califano, Alicia Noemí
Santos, María Victoria
Zaritzky, Noemí Elisabet
Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species
topic_facet Ciencias Exactas
Thermal inactivation kinetics
Lipoxygenase
Peroxidase
Precooked frozen Brassicas
Quality attributes
description Thermal inactivation of peroxidase (POD) and lipoxygenase (LOX), both enzymes present in broccoli and Brussels sprouts, is required before freezing, to obtain high-quality precooked frozen vegetables. Rate constants of a 1st-order biphasic model for the heat-labile and heat-resistant POD and LOX isoenzymes were determined at different temperatures (75, 80, and 90 °C) and the corresponding activation energies were estimated using nonlinear regressions. In the case of Brussels sprouts, the activation energies for the resistant and labile fractions were 56.3 and 62.5 kJ/mol for POD and 63.7 and 65.8 kJ/mol for LOX, respectively. For Brussels sprouts, different precooking times were tested to analyze the effect of residual enzyme activity on quality parameters and sensory attributes, after a frozen storage of 4 mo at −20 °C. A significant reactivation of enzyme activity after frozen storage was observed (especially in the case of POD) for short precooking times (<6 min) leading to low-quality parameters at the interior zone of the vegetable. A precooking time of 6 min at 90 °C allowed an adequate inactivation of LOX and POD obtaining a high-quality final frozen vegetable. A sensory analysis confirmed the global acceptability of the product. The obtained results are relevant to define the precooking stage conditions in the production of frozen cruciferous vegetables.
format Articulo
Articulo
author Pérez Calderón, John Freddy
Califano, Alicia Noemí
Santos, María Victoria
Zaritzky, Noemí Elisabet
author_facet Pérez Calderón, John Freddy
Califano, Alicia Noemí
Santos, María Victoria
Zaritzky, Noemí Elisabet
author_sort Pérez Calderón, John Freddy
title Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species
title_short Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species
title_full Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species
title_fullStr Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species
title_full_unstemmed Kinetic Parameters for the Thermal Inactivation of Peroxidase and Lipoxygenase in Precooked Frozen Brassica Species
title_sort kinetic parameters for the thermal inactivation of peroxidase and lipoxygenase in precooked frozen brassica species
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/103096
https://onlinelibrary.wiley.com/doi/10.1111/1750-3841.13717
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