A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders

Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn’s disease (CD) and ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence and affinity for b-galactosides, are critical modulators of the inf...

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Autores principales: Papa Gobbi, Rodrigo, De Francesco, Pablo Nicolás, Bondar, Constanza María, Muglia, Cecilia Isabel, Chirdo, Fernando Gabriel, Rumbo, Martín, Rocca, Andrés, Toscano, Marta A., Sambuelli, Alicia, Rabinovich, Gabriel A., Docena, Guillermo Horacio
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2016
Materias:
Ciencias Médicas
Inflammatory bowel diseases
Galectins
Crohn disease
Ulcerative colitis
Biomarkers
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/103068
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-103068
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Inflammatory bowel diseases
Galectins
Crohn disease
Ulcerative colitis
Biomarkers
spellingShingle Ciencias Médicas
Inflammatory bowel diseases
Galectins
Crohn disease
Ulcerative colitis
Biomarkers
Papa Gobbi, Rodrigo
De Francesco, Pablo Nicolás
Bondar, Constanza María
Muglia, Cecilia Isabel
Chirdo, Fernando Gabriel
Rumbo, Martín
Rocca, Andrés
Toscano, Marta A.
Sambuelli, Alicia
Rabinovich, Gabriel A.
Docena, Guillermo Horacio
A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
topic_facet Ciencias Médicas
Inflammatory bowel diseases
Galectins
Crohn disease
Ulcerative colitis
Biomarkers
description Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn’s disease (CD) and ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence and affinity for b-galactosides, are critical modulators of the inflammatory response. However, the relevance of the galectin network in the pathogenesis of human IBD has not yet been explored. Here, we analyzed the expression of relevant members of the galectin family in intestinal biopsies, and identified their contribution as novel mucosal markers in IBD. Colonic biopsies were obtained from 59 IBD patients (22 CD and 37 UC), 9 patients with gut rejection after transplantation, 8 adult celiac patients, and 32 non-IBD donors. Galectin mRNA expression was analyzed by RT-PCR and qPCR using specific primers for individual galectins. A linear discriminant analysis (LDA) was used to analyze galectin expression in individual intestinal samples. Expression of common mucosalassociated galectins (Gal-1, 23, 24, 29) is dysregulated in inflamed tissues of IBD patients compared with noninflamed IBD or control samples. LDA discriminated between different inflammation grades in active IBD and showed that remission IBD samples were clusterized with control samples. Galectin profiling could not distinguish CD and UC. Furthermore, inflamed IBD was discriminated from inflamed tissue of rejected gut in transplanted patients and duodenum of celiac patients, which could not be distinguished from control duodenum samples. The integrative analysis of galectins discriminated IBD from other intestinal inflammatory conditions and could be used as potential mucosal biomarker.
format Articulo
Preprint
author Papa Gobbi, Rodrigo
De Francesco, Pablo Nicolás
Bondar, Constanza María
Muglia, Cecilia Isabel
Chirdo, Fernando Gabriel
Rumbo, Martín
Rocca, Andrés
Toscano, Marta A.
Sambuelli, Alicia
Rabinovich, Gabriel A.
Docena, Guillermo Horacio
author_facet Papa Gobbi, Rodrigo
De Francesco, Pablo Nicolás
Bondar, Constanza María
Muglia, Cecilia Isabel
Chirdo, Fernando Gabriel
Rumbo, Martín
Rocca, Andrés
Toscano, Marta A.
Sambuelli, Alicia
Rabinovich, Gabriel A.
Docena, Guillermo Horacio
author_sort Papa Gobbi, Rodrigo
title A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
title_short A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
title_full A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
title_fullStr A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
title_full_unstemmed A galectin-specific signature in the gut delineates Crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
title_sort galectin-specific signature in the gut delineates crohn’s disease and ulcerative colitis from other human inflammatory intestinal disorders
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/103068
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