Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells

In previous studies have been reported a controversy between the reactivity of mono and polyclonal antibodies in different cells populations of the mouse. In this study we focus on the immunoreactivity of the monoclonal antibody VEGF (C-1) in order to analyze its expression in two cell populations o...

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Autores principales: Errecalde, Ana Lía, Inda, Ana María, García, Marcela Nilda, Andrini, Laura Beatriz, García, Adriana L., Furnus, Cecilia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Ciencias Médicas
mouse; regeneration liver; hepatocarcinoma; VEGF
experimentación animal
sustancia cancerígena
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/10287
http://revista.med.unlp.edu.ar/archivos/200411/expresion%20vascular.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-10287
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
mouse; regeneration liver; hepatocarcinoma; VEGF
experimentación animal
sustancia cancerígena
spellingShingle Ciencias Médicas
mouse; regeneration liver; hepatocarcinoma; VEGF
experimentación animal
sustancia cancerígena
Errecalde, Ana Lía
Inda, Ana María
García, Marcela Nilda
Andrini, Laura Beatriz
García, Adriana L.
Furnus, Cecilia Cristina
Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
topic_facet Ciencias Médicas
mouse; regeneration liver; hepatocarcinoma; VEGF
experimentación animal
sustancia cancerígena
description In previous studies have been reported a controversy between the reactivity of mono and polyclonal antibodies in different cells populations of the mouse. In this study we focus on the immunoreactivity of the monoclonal antibody VEGF (C-1) in order to analyze its expression in two cell populations of regenerating mouse liver (hepatocytes and endothelial cells) after partial hepatectomy, and two transplanted hepatocarcinomas (ES2 and SS1K). C3H/S male mice were divided into two groups and kept under standard conditions for circadian periodicity analysis, one group were transplanted with the tumors into the subcutaneous tissue of the animal flank and the other group were subjected to a partial hepatectomy (70 %). The animals were killed by decapitation and exsanguination. Samples of regeneration liver, ES2 and SS1K tumors were processed for histology and immunostained with VEGF (C-1) mouse monoclonal IgG2a antibody. The results showed strongly immunopositivity reaction for VEGF either in hepatocytes or endothelial cells of mouse regenerating liver. We also demonstrated positive immunoreaction in both malignant tumors ES2 and SS1K endothelial and parenchymal cells. We conclude that the use of VEGF (C-1) mouse monoclonal IgG2a antibody can be applied successfully in experimental design leading to study processes involved in mice’s angiogenesis
format Articulo
Articulo
author Errecalde, Ana Lía
Inda, Ana María
García, Marcela Nilda
Andrini, Laura Beatriz
García, Adriana L.
Furnus, Cecilia Cristina
author_facet Errecalde, Ana Lía
Inda, Ana María
García, Marcela Nilda
Andrini, Laura Beatriz
García, Adriana L.
Furnus, Cecilia Cristina
author_sort Errecalde, Ana Lía
title Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
title_short Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
title_full Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
title_fullStr Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
title_full_unstemmed Expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
title_sort expression of vascular endothelial growth factor in normal and tumoral liver mouse cells
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/10287
http://revista.med.unlp.edu.ar/archivos/200411/expresion%20vascular.pdf
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bdutipo_str Repositorios
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