Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model

Overconsumption of palatable energy-dense foods has negative health implications and it is associated with obesity andseveral eating disorders. Currently, little is known about the neuronal circuitries activated by the acute ingestion of arewarding stimulus. Here, we used a combination of immunohist...

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Autores principales: Valdivia Torres, Lesly Spring, Patrone, Anabela, Reynaldo, Mirta Beatriz, Perelló, Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
Biología
Ciencias Médicas
Food intake
Ventro tegmental area
Food reward
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/102620
https://ri.conicet.gov.ar/11336/32302
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0087478
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-102620
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Ciencias Médicas
Food intake
Ventro tegmental area
Food reward
spellingShingle Ciencias Exactas
Biología
Ciencias Médicas
Food intake
Ventro tegmental area
Food reward
Valdivia Torres, Lesly Spring
Patrone, Anabela
Reynaldo, Mirta Beatriz
Perelló, Mario
Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model
topic_facet Ciencias Exactas
Biología
Ciencias Médicas
Food intake
Ventro tegmental area
Food reward
description Overconsumption of palatable energy-dense foods has negative health implications and it is associated with obesity andseveral eating disorders. Currently, little is known about the neuronal circuitries activated by the acute ingestion of arewarding stimulus. Here, we used a combination of immunohistochemistry, pharmacology and neuronal tracing analysesto examine the role of the mesolimbic system in general, and the orexin neurons in particular, in a simple experimental testin which naı ̈ve mice are allowed to spontaneously eat a pellet of a high fat diet (HFD) for 2 h. We found that acute HFDactivates c-Fos expression in several reward-related brain areas, including the ventral tegmental area (VTA), nucleusaccumbens, central amygdala and lateral hypothalamic area. We also found that: i- HFD-mediated orosensory stimulationwas required for the mesolimbic pathway activation, ii- acute HFD differentially activates dopamine neurons of theparanigral, parabrachial pigmented and interfascicular sub-regions of the VTA, and iii- orexin neurons of the lateralhypothalamic area are responsive to acute HFD. Moreover, orexin signaling blockade, with the orexin 1 receptor antagonistSB-334867, reduces acute HFD consumption and c-Fos induction in the VTA but not in the other mesolimbic nuclei understudy. Finally, we found that most orexin neurons responsive to acute HFD innervate the VTA. Our results show that acuteHFD consumption recruits the mesolimbic system and that the full manifestation of this eating behavior requires theactivation of orexin signaling.
format Articulo
Articulo
author Valdivia Torres, Lesly Spring
Patrone, Anabela
Reynaldo, Mirta Beatriz
Perelló, Mario
author_facet Valdivia Torres, Lesly Spring
Patrone, Anabela
Reynaldo, Mirta Beatriz
Perelló, Mario
author_sort Valdivia Torres, Lesly Spring
title Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model
title_short Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model
title_full Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model
title_fullStr Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model
title_full_unstemmed Acute High Fat Diet Consumption Activates the Mesolimbic Circuit and Requires Orexin Signaling in a Mouse Model
title_sort acute high fat diet consumption activates the mesolimbic circuit and requires orexin signaling in a mouse model
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/102620
https://ri.conicet.gov.ar/11336/32302
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0087478
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