Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction

Surface-layers (S-layers) are macromolecular paracrystalline arrays of proteins or glycoproteins that can self-assemble into 2-dimensional semi-permeable meshworks to overlay the cell surface of many bacteria and archaea. They usually assemble into lattices with oblique, square or hexagonal symmetry...

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Detalles Bibliográficos
Autores principales: Mobili, Pablo, Serradell, María de los Ángeles, Mayer, Claudine, Arluison, Véronique, Gómez Zavaglia, Andrea
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Bioquímica
Protein-self-assembly
Metal-ions biosorption
Biomineralization
Nanobiotechnology
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/102454
https://ri.conicet.gov.ar/11336/10645
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-102454
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Protein-self-assembly
Metal-ions biosorption
Biomineralization
Nanobiotechnology
spellingShingle Bioquímica
Protein-self-assembly
Metal-ions biosorption
Biomineralization
Nanobiotechnology
Mobili, Pablo
Serradell, María de los Ángeles
Mayer, Claudine
Arluison, Véronique
Gómez Zavaglia, Andrea
Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction
topic_facet Bioquímica
Protein-self-assembly
Metal-ions biosorption
Biomineralization
Nanobiotechnology
description Surface-layers (S-layers) are macromolecular paracrystalline arrays of proteins or glycoproteins that can self-assemble into 2-dimensional semi-permeable meshworks to overlay the cell surface of many bacteria and archaea. They usually assemble into lattices with oblique, square or hexagonal symmetry and serve as an interface between the bacterial cell and the environment. Isolated S-layers can recrystallize into two-dimensional regular arrays in suspension or on various surfaces, thus being an appropriate material for several bionanotechnological purposes. Promising applications of S-layers include their use as biotemplates for the capture of metal ions or the synthesis of metal nanoclusters. Considering the use of S-layers as biotemplates for the organization of metal ions or metallic nanoclusters, research on potential of surface layer proteins (SLP) and metals can be understood as an interdisciplinary field, in which different biophysical techniques supply complementary information. In this review, we discuss the SLP as native or engineered “bottom-up” building blocks for metal immobilization structures. We also describe the biophysical techniques used to analyze metal binding properties as well as the information obtained from the investigation of these structures.
format Articulo
Articulo
author Mobili, Pablo
Serradell, María de los Ángeles
Mayer, Claudine
Arluison, Véronique
Gómez Zavaglia, Andrea
author_facet Mobili, Pablo
Serradell, María de los Ángeles
Mayer, Claudine
Arluison, Véronique
Gómez Zavaglia, Andrea
author_sort Mobili, Pablo
title Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction
title_short Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction
title_full Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction
title_fullStr Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction
title_full_unstemmed Biophysical Methods for the Elucidation of the S-Layer Proteins/Metal Interaction
title_sort biophysical methods for the elucidation of the s-layer proteins/metal interaction
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/102454
https://ri.conicet.gov.ar/11336/10645
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AT arluisonveronique biophysicalmethodsfortheelucidationoftheslayerproteinsmetalinteraction
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