Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia
It is known that alternating magnetic field applications on eukaryotic cells loaded with single domain iron oxide nanoparticles result in high hyperthermic citotoxicity leading to cell dead. Although magnetic hyperthermia therapy for cancer tumours is being developed under this idea, some <i>i...
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| Autores principales: | , , , , , , , , , |
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| Formato: | Articulo Preprint |
| Lenguaje: | Inglés |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/102397 https://ri.conicet.gov.ar/11336/48888 https://pubs.acs.org/doi/full/10.1021/acs.jpcc.5b12330 |
| Aporte de: |
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I19-R120-10915-102397 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Física Bioquímica magnetic hyperthermia intracellular heating iron-oxide nanoparticles human HSP70B promoter enhanced green fluorescence protein radio frequency magnetic field |
| spellingShingle |
Ciencias Exactas Física Bioquímica magnetic hyperthermia intracellular heating iron-oxide nanoparticles human HSP70B promoter enhanced green fluorescence protein radio frequency magnetic field Sousa, María Elisa Liliana de Carrea, Alejandra Mendoza Zélis, Pedro Muraca, Diego Mykhaylyk, Olga Sosa, Yolanda Elena Goya, Rodolfo Gustavo Sánchez, Francisco Homero Dewey, Ricardo Alfredo Fernández van Raap, Marcela Beatriz Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia |
| topic_facet |
Ciencias Exactas Física Bioquímica magnetic hyperthermia intracellular heating iron-oxide nanoparticles human HSP70B promoter enhanced green fluorescence protein radio frequency magnetic field |
| description |
It is known that alternating magnetic field applications on eukaryotic cells loaded with single domain iron oxide nanoparticles result in high hyperthermic citotoxicity leading to cell dead. Although magnetic hyperthermia therapy for cancer tumours is being developed under this idea, some <i>in vitro</i> assays have shown controversial results indicating that alternating magnetic field triggers large apoptotic effect without significant culture-temperature increase. In agreement with these observations a huge lowering in nanoparticle specific heating rates, when going from the colloidal suspension to cell endosomes, together with cell death, has been reported. Here, we propose a new methodology to determine the occurrence of local heating in cells when alternating magnetic fields in the radiofrequency field range are applied to cell cultures holding very low iron oxide concentrations, being these concentrations insufficient to produce a global cell-culture temperature increase up to therapeutic values. To this end, human lung adenocarcinoma cells (A549 cell line) were transduced with a lentiviral vector encoding the expression of the enhanced green fluorescence protein, EGFP, under the action of the inducible human heat shock protein 70B promoter. This modified A549 cell line was incubated with aqueous suspensions of magnetite core nanoparticles (uncoated or covered with coating agents like citric acid or silicon oxide), and exposed to radiofrequency fields. The application of an alternating magnetic field to cell cultures loaded with nanoparticles resulted in no global temperature increase but EGFP expression. Stress-inducible gene expression scales with uptake and nanoparticle properties like saturation magnetization and heat dissipation efficiency. Our analysis demonstrates that EGFP expression is linked to a localized intracellular temperature increase. |
| format |
Articulo Preprint |
| author |
Sousa, María Elisa Liliana de Carrea, Alejandra Mendoza Zélis, Pedro Muraca, Diego Mykhaylyk, Olga Sosa, Yolanda Elena Goya, Rodolfo Gustavo Sánchez, Francisco Homero Dewey, Ricardo Alfredo Fernández van Raap, Marcela Beatriz |
| author_facet |
Sousa, María Elisa Liliana de Carrea, Alejandra Mendoza Zélis, Pedro Muraca, Diego Mykhaylyk, Olga Sosa, Yolanda Elena Goya, Rodolfo Gustavo Sánchez, Francisco Homero Dewey, Ricardo Alfredo Fernández van Raap, Marcela Beatriz |
| author_sort |
Sousa, María Elisa Liliana de |
| title |
Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia |
| title_short |
Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia |
| title_full |
Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia |
| title_fullStr |
Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia |
| title_full_unstemmed |
Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia |
| title_sort |
stress-induced gene expression sensing intracellular heating triggered by magnetic hyperthermia |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/102397 https://ri.conicet.gov.ar/11336/48888 https://pubs.acs.org/doi/full/10.1021/acs.jpcc.5b12330 |
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