Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis
Spermatogenesis is a highly regulated process that involves both mitotic and meiotic divisions, as well as cellular differentiation to yield mature spermatozoa from undifferentiated germinal stem cells. Although <i>Gpat2</i> was originally annotated as a glycerol-3-phospate acyltransfera...
Guardado en:
| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2015
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/102388 https://ri.conicet.gov.ar/11336/48886 http://www.biochemj.org/content/early/2015/08/12/BJ20150730.long |
| Aporte de: |
| id |
I19-R120-10915-102388 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Medicina Ciencias Exactas epigenetic regulation GPAT2 Meiosis retinoic acid |
| spellingShingle |
Medicina Ciencias Exactas epigenetic regulation GPAT2 Meiosis retinoic acid García Fabiani, María Belén Montanaro, Mauro Aldo Lacunza, Ezequiel Cattaneo, Elizabeth Renee Coleman, Rosalind A. Pellón Maisón, Magalí González Baró, María del Rosario Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| topic_facet |
Medicina Ciencias Exactas epigenetic regulation GPAT2 Meiosis retinoic acid |
| description |
Spermatogenesis is a highly regulated process that involves both mitotic and meiotic divisions, as well as cellular differentiation to yield mature spermatozoa from undifferentiated germinal stem cells. Although <i>Gpat2</i> was originally annotated as a glycerol-3-phospate acyltransferase by sequence homology to <i>Gpat1</i>, GPAT2 is highly expressed in testis but not in lipogenic tissues and is not up-regulated during adipocyte differentiation. New data show that GPAT2 is required for the synthesis of piRNAs, a group of small RNAs that protect the germ cell genome from retrotransposable elements. In order to understand the relationship between GPAT2 and its role in the testis, we focused on <i>Gpat2</i> expression during the first wave of mouse spermatogenesis. <i>Gpat2</i> expression was analyzed by qPCR, <i>in situ</i> hybridization, immunohistochemistry and Western blot. <i>Gpat2</i> mRNA content and protein expression were maximal at 15 dpp and restricted to pachytene spermatocytes. To achieve this transient expression, both epigenetic mechanisms and <i>trans</i>-acting factors are involved. <i>In vitro</i> assays showed that <i>Gpat2</i> expression correlates with DNA demethylation and histone acetylation and that it is up-regulated by retinoic acid. Epigenetic regulation by DNA methylation was confirmed <i>in vivo</i> in germ cells by bisulfite sequencing of the <i>Gpat2</i> promoter. Consistent with the initiation of meiosis at 11 dpp, methylation decreased dramatically. Thus, <i>Gpat2</i> is expressed at a specific stage of spermatogenesis, consistent with piRNA synthesis and meiosis I prophase, and its on-off expression pattern responds predominantly to epigenetic modifications. |
| format |
Articulo Articulo |
| author |
García Fabiani, María Belén Montanaro, Mauro Aldo Lacunza, Ezequiel Cattaneo, Elizabeth Renee Coleman, Rosalind A. Pellón Maisón, Magalí González Baró, María del Rosario |
| author_facet |
García Fabiani, María Belén Montanaro, Mauro Aldo Lacunza, Ezequiel Cattaneo, Elizabeth Renee Coleman, Rosalind A. Pellón Maisón, Magalí González Baró, María del Rosario |
| author_sort |
García Fabiani, María Belén |
| title |
Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| title_short |
Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| title_full |
Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| title_fullStr |
Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| title_full_unstemmed |
Methylation of the <i>Gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| title_sort |
methylation of the <i>gpat2</i> promoter regulates transient expression during mouse spermatogenesis |
| publishDate |
2015 |
| url |
http://sedici.unlp.edu.ar/handle/10915/102388 https://ri.conicet.gov.ar/11336/48886 http://www.biochemj.org/content/early/2015/08/12/BJ20150730.long |
| work_keys_str_mv |
AT garciafabianimariabelen methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis AT montanaromauroaldo methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis AT lacunzaezequiel methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis AT cattaneoelizabethrenee methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis AT colemanrosalinda methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis AT pellonmaisonmagali methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis AT gonzalezbaromariadelrosario methylationoftheigpat2ipromoterregulatestransientexpressionduringmousespermatogenesis |
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Repositorios |
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1764820441705218051 |