Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization

Levofloxacin (LV) is a hydrophilic broad-spectrum antibiotic commonly used in pulmonary treatment against recurrent infections of Pseudomonas aeruginosa, and particularly in cystic fibrosis (CF) disease. In order to study feasible carriers for LV, solid lipid nanoparticles (SLN) of myristyl myristat...

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Autores principales:	Islan, Germán Abel, Cortez Tornello, Pablo Roberto, Abraham, Gustavo Abel, Duran, Nelson, Castro, Guillermo Raúl
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2016
Materias:	Química Cystic fibrosis DNase Levofloxacin Nanostructured lipid carriers Pulmonary delivery Solid lipid nanoparticles
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/102264 https://ri.conicet.gov.ar/11336/40036
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-102264
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química Cystic fibrosis DNase Levofloxacin Nanostructured lipid carriers Pulmonary delivery Solid lipid nanoparticles
spellingShingle	Química Cystic fibrosis DNase Levofloxacin Nanostructured lipid carriers Pulmonary delivery Solid lipid nanoparticles Islan, Germán Abel Cortez Tornello, Pablo Roberto Abraham, Gustavo Abel Duran, Nelson Castro, Guillermo Raúl Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization
topic_facet	Química Cystic fibrosis DNase Levofloxacin Nanostructured lipid carriers Pulmonary delivery Solid lipid nanoparticles
description	Levofloxacin (LV) is a hydrophilic broad-spectrum antibiotic commonly used in pulmonary treatment against recurrent infections of Pseudomonas aeruginosa, and particularly in cystic fibrosis (CF) disease. In order to study feasible carriers for LV, solid lipid nanoparticles (SLN) of myristyl myristate were prepared by the ultrasonication method in the presence of Pluronic®F68 under different experimental conditions and characterized by dynamic light scattering, optical, transmission and scanning electron microscopy for size and morphology. Alternatively, nanostructured lipid carriers (NLCs) were developed to improve LV encapsulation and storage. SLN showed 20.1 ± 1.4% LV encapsulation efficiency, while the NLCs encapsulated 55.9 ± 1.6% LV. NLC formulation exhibited a more controlled release profile than SLN formulation, but both showed a biphasic drug release pattern with burst release at the first 5 h and prolonged release afterwards, demonstrated by in vitro tests. The hydrodynamic average diameter and zeta potential of NLC were 182.6 ± 3.2 nm and -10.2 ± 0.2 mV, respectively, and were stable for at least 3 months. Additionally, DNase type I was incorporated into the formulations as a "smart" component, since the enzyme could help to decrease the viscoelasticity found in the lungs of CF patients and improves the antibiotic diffusion. FTIR, XRD, DSC, TGA and nitrogen adsorption isotherms of the nanoparticles indicate the presence of the loads in a noncrystalline state. The developed formulation showed an active antimicrobial activity against P. aeruginosa and even against other opportunistic pathogens such as Staphylococcus aureus. The presence of LV-loaded NLCs reduced the formation of a bacterial biofilm, which highlighted the significance of the nanodevice as a new alternative for CF treatment.
format	Articulo Preprint
author	Islan, Germán Abel Cortez Tornello, Pablo Roberto Abraham, Gustavo Abel Duran, Nelson Castro, Guillermo Raúl
author_facet	Islan, Germán Abel Cortez Tornello, Pablo Roberto Abraham, Gustavo Abel Duran, Nelson Castro, Guillermo Raúl
author_sort	Islan, Germán Abel
title	Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization
title_short	Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization
title_full	Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization
title_fullStr	Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization
title_full_unstemmed	Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization
title_sort	smart lipid nanoparticles containing levofloxacin and dnase for lung delivery: design and characterization
publishDate	2016
url	http://sedici.unlp.edu.ar/handle/10915/102264 https://ri.conicet.gov.ar/11336/40036
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Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery: design and characterization

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