Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella

Aiming to combine the flexibility of Brucella lumazine synthase (BLS) to adapt different protein domains in a decameric structure and the capacity of BLS and flagellin to enhance the immunogenicity of peptides that are linked to their structure, we generated a chimeric protein (BLS-FliC131) by fusin...

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Detalles Bibliográficos
Autores principales: Hiriart, Yanina, Rossi, Andrés Hugo, Biedma, Marina Elizabeth, Errea, Agustina Juliana, Moreno, Griselda Noemí, Cayet, D., Rinaldi, Jimena Julieta, Blancá, Bruno Martín, Sirard, J.C., Goldbaum, Fernando Alberto, Berguer, Paula Mercedes, Rumbo, Martín
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Bioquímica
BLS
Flagellin
Scaffold
TLR5
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101811
https://ri.conicet.gov.ar/11336/29771
http://onlinelibrary.wiley.com/doi/10.1002/pro.3151/full
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101811
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bioquímica
BLS
Flagellin
Scaffold
TLR5
spellingShingle Biología
Bioquímica
BLS
Flagellin
Scaffold
TLR5
Hiriart, Yanina
Rossi, Andrés Hugo
Biedma, Marina Elizabeth
Errea, Agustina Juliana
Moreno, Griselda Noemí
Cayet, D.
Rinaldi, Jimena Julieta
Blancá, Bruno Martín
Sirard, J.C.
Goldbaum, Fernando Alberto
Berguer, Paula Mercedes
Rumbo, Martín
Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella
topic_facet Biología
Bioquímica
BLS
Flagellin
Scaffold
TLR5
description Aiming to combine the flexibility of Brucella lumazine synthase (BLS) to adapt different protein domains in a decameric structure and the capacity of BLS and flagellin to enhance the immunogenicity of peptides that are linked to their structure, we generated a chimeric protein (BLS-FliC131) by fusing flagellin from Salmonella in the N-termini of BLS. The obtained protein was recognized by anti-flagellin and anti-BLS antibodies, keeping the oligomerization capacity of BLS, without affecting the folding of the monomeric protein components determined by circular dichroism. Furthermore, the thermal stability of each fusion partner is conserved, indicating that the interactions that participate in its folding are not affected by the genetic fusion. Besides, either in vitro or in vivo using TLR5-deficient animals we could determine that BLS-FliC131 retains the capacity of triggering TLR5. The humoral response against BLS elicited by BLS-FliC131 was stronger than the one elicited by equimolar amounts of BLS + FliC. Since BLS scaffold allows the generation of hetero-decameric structures, we expect that flagellin oligomerization on this protein scaffold will generate a new vaccine platform with enhanced capacity to activate immune responses
format Articulo
Preprint
author Hiriart, Yanina
Rossi, Andrés Hugo
Biedma, Marina Elizabeth
Errea, Agustina Juliana
Moreno, Griselda Noemí
Cayet, D.
Rinaldi, Jimena Julieta
Blancá, Bruno Martín
Sirard, J.C.
Goldbaum, Fernando Alberto
Berguer, Paula Mercedes
Rumbo, Martín
author_facet Hiriart, Yanina
Rossi, Andrés Hugo
Biedma, Marina Elizabeth
Errea, Agustina Juliana
Moreno, Griselda Noemí
Cayet, D.
Rinaldi, Jimena Julieta
Blancá, Bruno Martín
Sirard, J.C.
Goldbaum, Fernando Alberto
Berguer, Paula Mercedes
Rumbo, Martín
author_sort Hiriart, Yanina
title Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella
title_short Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella
title_full Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella
title_fullStr Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella
title_full_unstemmed Characterization of structural and immunological properties of a fusion protein between flagellin from Salmonella and lumazine synthase from Brucella
title_sort characterization of structural and immunological properties of a fusion protein between flagellin from salmonella and lumazine synthase from brucella
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/101811
https://ri.conicet.gov.ar/11336/29771
http://onlinelibrary.wiley.com/doi/10.1002/pro.3151/full
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