Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
Aims: To analyse the effect of <i>Enterococcus faecalis</i> CECT7121 on intestinalepithelial cells (IECs) and its effects on the mucosal immune response. Methods and Results: <i>Enterococcus faecalis</i> CECT7121 showed a high adhesioncapacity to completely and heterogeneous...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2016
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/101711 https://ri.conicet.gov.ar/11336/47117 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13226 |
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I19-R120-10915-101711 |
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dspace |
| institution |
Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Medicina Ciencias Exactas adhesion to intestinal epithelial cells Enterococcus faecalis CECT7121 immunomodulation mucosal immune response probiotic activity |
| spellingShingle |
Medicina Ciencias Exactas adhesion to intestinal epithelial cells Enterococcus faecalis CECT7121 immunomodulation mucosal immune response probiotic activity Castro, Marisa Silvia Molina, Matías Alejandro Azpiroz, María Belén Díaz, Ailén Magalí Ponzio, R. Sparo, Mónica Delfina Manghi, Marcela Alejandra Canellada, Andrea Mercedes Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells |
| topic_facet |
Medicina Ciencias Exactas adhesion to intestinal epithelial cells Enterococcus faecalis CECT7121 immunomodulation mucosal immune response probiotic activity |
| description |
Aims: To analyse the effect of <i>Enterococcus faecalis</i> CECT7121 on intestinalepithelial cells (IECs) and its effects on the mucosal immune response.
Methods and Results: <i>Enterococcus faecalis</i> CECT7121 showed a high adhesioncapacity to completely and heterogeneously differentiated human intestinalepithelial cell line (Caco-2 cells). In addition, the contact of this bacteriumwith Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA⁺ and IL-6⁺ cells in the small intestine, as well as theproduction of inflammatory cytokines (TNFa, IL-6 and IL-12) in the gut, wasdetermined after intragastric inoculation of <i>Ent. faecalis</i> CECT7121 in BALB/cmice. The administration of <i>Ent. faecalis</i> CECT7121 increased the number ofIgA⁺ cells in the intestinal lamina propria without modifying the percentage ofIL-6⁺ cells. No differences were observed in the cytokines measured in theintestinal extracts between probiotic-treated and control mice.
Conclusions: <i>Enterococcus faecalis</i> CECT7121 stimulates local mucosalimmunity and adheres to IECs without inducing inflammatory signals.
Significance and Impact of the Study: Our results indicate that, apart from itsalready reported systemic immune activity, <i>Ent. faecalis</i> CECT7121 has amodulatory effect at a local level. |
| format |
Articulo Articulo |
| author |
Castro, Marisa Silvia Molina, Matías Alejandro Azpiroz, María Belén Díaz, Ailén Magalí Ponzio, R. Sparo, Mónica Delfina Manghi, Marcela Alejandra Canellada, Andrea Mercedes |
| author_facet |
Castro, Marisa Silvia Molina, Matías Alejandro Azpiroz, María Belén Díaz, Ailén Magalí Ponzio, R. Sparo, Mónica Delfina Manghi, Marcela Alejandra Canellada, Andrea Mercedes |
| author_sort |
Castro, Marisa Silvia |
| title |
Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells |
| title_short |
Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells |
| title_full |
Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells |
| title_fullStr |
Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells |
| title_full_unstemmed |
Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells |
| title_sort |
probiotic activity of <i>enterococcus faecalis</i> cect7121: effects on mucosal immunity and intestinal epithelial cells |
| publishDate |
2016 |
| url |
http://sedici.unlp.edu.ar/handle/10915/101711 https://ri.conicet.gov.ar/11336/47117 https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13226 |
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