Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study

Sphingomyelins (SM) and phosphatidylcholines (PC) are major lipid classes in the external plasma membrane leaflet of mammalian cells. A preferential interaction between SM and cholesterol (Cho) in both cell and model membranes has been proposed as central for the formation of Cho- and SM-rich domain...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maté, Sabina María, Vázquez, Romina Florencia, Pavinatto, Felippe J., Daza Millone, María Antonieta, Herlax, Vanesa Silvana, Bakás, Laura Susana, Oliveira, Osvaldo N., Vela, María Elena
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Mammalian membranes
PM-IRRAS
Gibbs monolayers
Alfa hemolysin a
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101692
https://ri.conicet.gov.ar/11336/64227
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101692
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Mammalian membranes
PM-IRRAS
Gibbs monolayers
Alfa hemolysin a
spellingShingle Ciencias Exactas
Mammalian membranes
PM-IRRAS
Gibbs monolayers
Alfa hemolysin a
Maté, Sabina María
Vázquez, Romina Florencia
Pavinatto, Felippe J.
Daza Millone, María Antonieta
Herlax, Vanesa Silvana
Bakás, Laura Susana
Oliveira, Osvaldo N.
Vela, María Elena
Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study
topic_facet Ciencias Exactas
Mammalian membranes
PM-IRRAS
Gibbs monolayers
Alfa hemolysin a
description Sphingomyelins (SM) and phosphatidylcholines (PC) are major lipid classes in the external plasma membrane leaflet of mammalian cells. A preferential interaction between SM and cholesterol (Cho) in both cell and model membranes has been proposed as central for the formation of Cho- and SM-rich domains in membranes. In this context, the relevance of the SM hydrophobic moiety on its interaction with Cho for domain stabilization has been investigated by our group (1-2). We report here on the effects of sphingomyelin structure on the orientational and conformational properties of monolayers of pure lipids and of two ternary lipid mixtures (DOPC/16:0SM/Cho and DOPC/24:1SM/Cho), which are relevant as mammalian cell membrane models. We investigated interchain interactions, hydrogen bonding, conformational and structural properties using in situ polarization modulated infrared reflection absorption spectroscopy (PM-IRRAS). Our results indicate that the particular properties conferred on sphingolipids by unsaturation have profound implications on membrane organization.Finally, we also explored the orientational and conformational changes in lipid monolayers of DOPC/16:0SM/Cho 2:1:1 after the adsorption/insertion of the active toxin HlyA and its unacylated nonhemolytic precursor ProHlyA, so as to complement our knowledge on the action mechanism of both proteins.
format Articulo
Comunicacion
author Maté, Sabina María
Vázquez, Romina Florencia
Pavinatto, Felippe J.
Daza Millone, María Antonieta
Herlax, Vanesa Silvana
Bakás, Laura Susana
Oliveira, Osvaldo N.
Vela, María Elena
author_facet Maté, Sabina María
Vázquez, Romina Florencia
Pavinatto, Felippe J.
Daza Millone, María Antonieta
Herlax, Vanesa Silvana
Bakás, Laura Susana
Oliveira, Osvaldo N.
Vela, María Elena
author_sort Maté, Sabina María
title Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study
title_short Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study
title_full Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study
title_fullStr Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study
title_full_unstemmed Orientational Properties of DOPC/SM/Cholesterol Mixtures: A PM-IRRAS Study
title_sort orientational properties of dopc/sm/cholesterol mixtures: a pm-irras study
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/101692
https://ri.conicet.gov.ar/11336/64227
work_keys_str_mv AT matesabinamaria orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT vazquezrominaflorencia orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT pavinattofelippej orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT dazamillonemariaantonieta orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT herlaxvanesasilvana orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT bakaslaurasusana orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT oliveiraosvaldon orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
AT velamariaelena orientationalpropertiesofdopcsmcholesterolmixturesapmirrasstudy
bdutipo_str Repositorios
_version_ 1764820442872283138

Ejemplares similares