Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease

Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator....

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Autores principales: Rodríguez, Fernanda Mariel, Salvetti, Natalia Raquel, Colombero, M., Stangaferro, M., Barbeito, Claudio Gustavo, Ortega, Hugo Héctor, Rey, Florencia
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Veterinarias
Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101683
https://ri.conicet.gov.ar/11336/6398
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101683
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
spellingShingle Ciencias Veterinarias
Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
Rodríguez, Fernanda Mariel
Salvetti, Natalia Raquel
Colombero, M.
Stangaferro, M.
Barbeito, Claudio Gustavo
Ortega, Hugo Héctor
Rey, Florencia
Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
topic_facet Ciencias Veterinarias
Cattle
Cystic ovarian disease
Insulin-like growth factor
Ovaries
description Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P menor 0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
format Articulo
Preprint
author Rodríguez, Fernanda Mariel
Salvetti, Natalia Raquel
Colombero, M.
Stangaferro, M.
Barbeito, Claudio Gustavo
Ortega, Hugo Héctor
Rey, Florencia
author_facet Rodríguez, Fernanda Mariel
Salvetti, Natalia Raquel
Colombero, M.
Stangaferro, M.
Barbeito, Claudio Gustavo
Ortega, Hugo Héctor
Rey, Florencia
author_sort Rodríguez, Fernanda Mariel
title Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_short Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_full Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_fullStr Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_full_unstemmed Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease
title_sort interaction between igf1 and igfbps in bovine cystic ovarian disease
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/101683
https://ri.conicet.gov.ar/11336/6398
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