Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves

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Leaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation of breakdown products to other plant organs. It is accompanied by increased production of reactive oxygen species (ROS) that are proposed to signal cell death, although both...

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Autores principales: Mayta, Martín Leonardo, Lodeyro, Anabella Fernanda, Guiamet, Juan José, Tognetti, Vanesa B., Melzer, Michael, Hajirezaei, Mohammad R., Carrillo, Néstor José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Agrarias
Chloroplasts
Flavodoxin
Reactive oxygen species
Redox poise
Senescence
Transgenic plants
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101460
https://ri.conicet.gov.ar/11336/88502
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-101460
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Chloroplasts
Flavodoxin
Reactive oxygen species
Redox poise
Senescence
Transgenic plants
spellingShingle Ciencias Agrarias
Chloroplasts
Flavodoxin
Reactive oxygen species
Redox poise
Senescence
Transgenic plants
Mayta, Martín Leonardo
Lodeyro, Anabella Fernanda
Guiamet, Juan José
Tognetti, Vanesa B.
Melzer, Michael
Hajirezaei, Mohammad R.
Carrillo, Néstor José
Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
topic_facet Ciencias Agrarias
Chloroplasts
Flavodoxin
Reactive oxygen species
Redox poise
Senescence
Transgenic plants
description Leaf senescence is a concerted physiological process involving controlled degradation of cellular structures and reallocation of breakdown products to other plant organs. It is accompanied by increased production of reactive oxygen species (ROS) that are proposed to signal cell death, although both the origin and the precise role of ROS in the execution of this developmental program are still poorly understood. To investigate the contribution of chloroplast-associated ROS to natural leaf senescence, we used tobacco plants expressing a plastid-targeted flavodoxin, an electron shuttle flavoprotein present in prokaryotes and algae. When expressed in plants, flavodoxin specifically prevents ROS formation in chloroplasts during stress situations. Senescence symptoms were significantly mitigated in these transformants, with decreased accumulation of chloroplastic ROS and differential preservation of chlorophylls, carotenoids, protein contents, cell and chloroplast structures, membrane integrity and cell viability. Flavodoxin also improved maintenance of chlorophyll-protein complexes, photosynthetic electron flow, CO2 assimilation, central metabolic routes and levels of bioactive cytokinins and auxins in aging leaves. Delayed induction of senescence-associated genes indicates that the entire genetic program of senescence was affected by flavodoxin. The results suggest that ROS generated in chloroplasts are involved in the regulation of natural leaf senescence.
format Articulo
Articulo
author Mayta, Martín Leonardo
Lodeyro, Anabella Fernanda
Guiamet, Juan José
Tognetti, Vanesa B.
Melzer, Michael
Hajirezaei, Mohammad R.
Carrillo, Néstor José
author_facet Mayta, Martín Leonardo
Lodeyro, Anabella Fernanda
Guiamet, Juan José
Tognetti, Vanesa B.
Melzer, Michael
Hajirezaei, Mohammad R.
Carrillo, Néstor José
author_sort Mayta, Martín Leonardo
title Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
title_short Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
title_full Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
title_fullStr Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
title_full_unstemmed Expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
title_sort expression of a plastid-targeted flavodoxin decreases chloroplast reactive oxygen species accumulation and delays senescence in aging tobacco leaves
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/101460
https://ri.conicet.gov.ar/11336/88502
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